Kaspaza-3
| Kaspaza-3 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Identifikatori | |||||||||
| EC broj | 3.4.22.56 | ||||||||
| CAS broj | 169592-56-7 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Kaspaza-3 (EC 3.4.22.56, CPP32, apopain, jama protein) je enzim.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Neophodno je prisustvo Asp ostatka u pozicijama P1 i P4. Preferentno dolazi do razlaganja sekvence Asp-Xaa-Xaa-Asp- sa hidrofobnim aminokiselinskim ostatkom u P2 poziciji i hidrofilnim aminokiselinskim ostatkom u P3, mada Val ili Ala takođe mogu da budu u toj poziciji
Kaspaza-3 je efektor/izvršilac kaspaze, kao što su i kaspaza-6 (EC 3.4.22.59) i kaspaza-7 (EC 3.4.22.60).
Reference[уреди | уреди извор]
- ^ Krebs, J.F., Srinivasan, A., Wong, A.M., Tomaselli, K.J., Fritz, L.C. and Wu, J.C. (2000). „Heavy membrane-associated caspase 3: identification, isolation, and characterization”. Biochemistry. 39: 16056—16063. PMID 11123933.
- ^ Li, H., Bergeron, L., Cryns, V., Pasternack, M.S., Zhu, H., Shi, L., Greenberg, A. and Yuan, J. (1997). „Activation of caspase-2 in apoptosis”. J. Biol. Chem. 272: 21010—21017. PMID 9261102.
- ^ Nicholson, D. & Thornberry, N.A. (2004). „Caspase-3 and caspase-7”. Ур.: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. Handbook of Proteolytic Enzymes (2nd изд.). London: Elsevier. стр. 1298—1302.
- ^ Fang, B., Boross, P.I., Tozser, J. and Weber, I.T. (2006). „Structural and kinetic analysis of caspase-3 reveals role for S5 binding site in substrate recognition”. J. Mol. Biol. 360: 654—666. PMID 16781734.
- ^ Chang, H.Y. & Yang, X. (2000). „Proteases for cell suicide: functions and regulation of caspases”. Microbiol. Mol. Biol. Rev. 64: 821—846. PMID 11104820.
- ^ Martin, S.J., Amarante-Mendes, G.P., Shi, L., Chuang, T.H., Casiano, C.A., O'Brien, G.A., Fitzgerald, P., Tan, E.M., Bokoch, G.M., Greenberg, A.H. and Green, D.R. (1996). „The cytotoxic cell protease granzyme B initiates apoptosis in a cell-free system by proteolytic processing and activation of the ICE/CED-3 family protease, CPP32, via a novel two-step mechanism”. EMBO J. 15: 2407—2416. PMID 8665848.
Literatura[уреди | уреди извор]
- Nicholson, D. & Thornberry, N.A. (2004). „Caspase-3 and caspase-7”. Ур.: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. Handbook of Proteolytic Enzymes (2nd изд.). London: Elsevier. стр. 1298—1302.
