Ent-kauren oksidaza
| Ent-kauren oksidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.78 | ||||||||
| CAS broj | 149565-67-3 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Ent-kauren oksidaza (EC 1.14.13.78, ent-kaurenska oksidaza) je enzim sa sistematskim imenom ent-kaur-16-en,NADPH:kiseonik oksidoreduktaza (hidroksilacija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- ent-kaur-16-en + 3 NADPH + 3 H+ + 3 O2 ent-kaur-16-en-19-oat + 3 NADP+ + 4H2O (sveukupna reakcija)
- (1a) ent-kaur-16-en + NADPH + H+ + O2 ent-kaur-16-en-19-ol + NADP+ + H2O
- (1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 ent-kaur-16-en-19-al + NADP+ + 2H2O
- (1c) ent-kaur-16-en-19-al + NADPH + O2 ent-kaur-16-en-19-oat + NADP+ + H2O
Za rad ovog enzima je neophodan citohrom P450. On katalizuje tri sukcesivne oksidacije 4-metil grupe ent-kaurena dajući kaurenoinsku kiselinu.
Reference[уреди | уреди извор]
- ^ Ashman, P.J., Mackenzie, A. and Bramley, P.M. (1990). „Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi”. Biochim. Biophys. Acta. 1036: 151—157. PMID 2223832.
- ^ Archer, C., Ashman, P.J., Hedden, P., Bowyer, J.R. and Bramley, P.M. (1992). „Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima”. Biochem. Soc. Trans. 20: 218—218. PMID 1397591.
- ^ Helliwell, C.A., Poole, A., Peacock, W.J. and Dennis, E.S. (1999). „Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis”. Plant Physiol. 119: 507—510. PMID 9952446.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- Ent-kaurene+oxidase на US National Library of Medicine Medical Subject Headings (MeSH)
