Gelanska lijaza
| Gelanska lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.2.25 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Gelanska lijaza (EC 4.2.2.25) je enzim sa sistematskim imenom gelan beta-D-glukopiranozil-(1->4)-D-glukopiranoziluronat lijaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Eliminativno razlaganje beta-D-glukopiranozil-(1->4)-beta-D-glukopiranoziluronatnih veza gelanske osnove uz odvajanje tetrasaharida koji sadrži 4-dezoksi-4,5-nezasićenu D-glukopiranoziluronsku kiselinu na neredukujućem kraju. Tetrasaharid formiran iz deacetilisanog gelana je beta-D-4-dezoksi-Delta4-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alfa-L-Rhap-(1->3)-beta-D-Glcp.
Ovaj enzim je veoma specifičan za gelan, i posebno za deacetilisani gelan.
Reference[уреди | уреди извор]
- ^ Hashimoto, W., Maesaka, K., Sato, N., Kimura, S., Yamamoto, K., Kumagai, H. and Murata, K. (1997). „Microbial system for polysaccharide depolymerization: enzymatic route for gellan depolymerization by Bacillus sp. GL1”. Arch. Biochem. Biophys. 339: 17—23. PMID 9056228.
- ^ Hashimoto, W., Sato, N., Kimura, S. and Murata, K. (1998). „Polysaccharide lyase: molecular cloning of gellan lyase gene and formation of the lyase from a huge precursor protein in Bacillus sp. GL1”. Arch. Biochem. Biophys. 354: 31—39. PMID 9633595.
- ^ Miyake, O., Kobayashi, E., Nankai, H., Hashimoto, W., Mikami, B. and Murata, K. (2004). „Posttranslational processing of polysaccharide lyase: maturation route for gellan lyase in Bacillus sp. GL1”. Arch. Biochem. Biophys. 422: 211—220. PMID 14759609.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Gellan+lyase на US National Library of Medicine Medical Subject Headings (MeSH)
