Lipoat—protein ligaza

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Lipoat—protein ligaza
Identifikatori
EC broj 2.7.7.63
CAS broj 144114-18-1
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Lipoat—protein ligaza (EC 2.7.7.63, LplA, lipoat proteinska ligaza, lipoat-proteinska ligaza A, LPL, LPL-B) je enzim sa sistematskim imenom ATP:lipoat adenililtransferaza.[1][2][3][4][5][6][7] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) ATP + lipoat \rightleftharpoons difosfat + lipoil-AMP
(2) lipoil-AMP + apoprotein \rightleftharpoons protein N6-(lipoil)lizin + AMP

Za rad ovog enzima je neophodan jon Mg2+.

Reference[уреди]

  1. ^ Morris, T.W., Reed, K.E. and Cronan, J.E., Jr. (1994). „Identification of the gene encoding lipoate-protein ligase A of Escherichia coli. Molecular cloning and characterization of the lplA gene and gene product“. J. Biol. Chem. 269: 16091-16100. PMID 8206909. 
  2. ^ Green, D.E., Morris, T.W., Green, J., Cronan, J.E., Jr. and Guest, J.R. (1995). „Purification and properties of the lipoate protein ligase of Escherichia coli“. Biochem. J. 309: 853-862. PMID 7639702. 
  3. ^ Zhao, X., Miller, J.R., Jiang, Y., Marletta, M.A. and Cronan, J.E. (2003). „Assembly of the covalent linkage between lipoic acid and its cognate enzymes“. Chem. Biol. 10: 1293-1302. PMID 14700636. 
  4. ^ Kim do, J., Kim, K.H., Lee, H.H., Lee, S.J., Ha, J.Y., Yoon, H.J. and Suh, S.W. (2005). „Crystal structure of lipoate-protein ligase A bound with the activated intermediate: insights into interaction with lipoyl domains“. J. Biol. Chem. 280: 38081-38089. PMID 16141198. 
  5. ^ Fujiwara, K., Toma, S., Okamura-Ikeda, K., Motokawa, Y., Nakagawa, A. and Taniguchi, H. (2005). „Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site“. J. Biol. Chem. 280: 33645-33651. PMID 16043486. 
  6. ^ Jordan, S.W. and Cronan, J.E., Jr. (1997). „A new metabolic link. The acyl carrier protein of lipid synthesis donates lipoic acid to the pyruvate dehydrogenase complex in Escherichia coli and mitochondria“. J. Biol. Chem. 272: 17903-17906. PMID 9218413. 
  7. ^ Perham, R.N. (2000). „Swinging arms and swinging domains in multifunctional enzymes: catalytic machines for multistep reactions“. Annu. Rev. Biochem. 69: 961-1004. PMID 10966480. 

Literatura[уреди]

Spoljašnje vezе[уреди]