Камфор 1,2-монооксигеназа
| Kamfor 1,2-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.15.2 | ||||||||
| CAS broj | 37256-81-8 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Kamfor 1,2-monooksigenaza (EC 1.14.15.2, 2,5-diketokamfan laktonizirajući enzim, kamforna ketolaktonaza I, ketolaktonaza I) je enzim sa sistematskim imenom (+)-kamfor,redukovani-rubredoksin:kiseonik oksidoreduktaza (1,2-laktonizacija).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju:
- (+)-bornan-2,5-dion + redukovani rubredoksin + O2 5-okso-1,2-kamfolid + oksidovani rubredoksin + H2O
Ovaj enzim je flavoprotein za čije dejstvo je neophodno Fe2+. On je izolovan iz Pseudomonas putida. Produkt se spontano konvertuje u [(1R)-5-okso-2,2,3-trimetilciklopent-3-enil]acetat.
Reference[уреди | уреди извор]
- ^ Conrad, H.E., DuBus, R., Namtvedt, M.J. and Gunsalus, I.C. (1965). „Mixed function oxidation. II. Separation and properties of the enzymes catalyzing camphor lactonizaton”. J. Biol. Chem. 240: 495—503. PMID 14253460.
- ^ Trudgill, P.W., DuBus, R. and Gunsalus, I.C. (1966). „Mixed function oxidation. VI. Purification of a tightly coupled electron transport complex in camphor lactonization”. J. Biol. Chem. 241: 4288—4290. PMID 4288652.
- ^ Yu, C.A. & Gunsalus, I.C. (1969). „Monoxygenases. VII. Camphor ketolactonase I and the role of three protein components”. J. Biol. Chem. 244: 6149—6152. PMID 4310834.
- ^ Taylor, D.G. & Trudgill, P.W. (1986). „Camphor revisited: studies of 2,5-diketocamphane 1,2-monooxygenase from Pseudomonas putida ATCC 17453”. J. Bacteriol. 165: 489—497. PMID 3944058.
- ^ Lounnas, V. & Wade, R.C. (1997). „Exceptionally stable salt bridges in cytochrome P450cam have functional roles”. Biochemistry. 36: 5402—5417. PMID 9154922.
- ^ Kadow, M., Sass, S., Schmidt, M. and Bornscheuer, U.T. (2011). „Recombinant expression and purification of the 2,5-diketocamphane 1,2-monooxygenase from the camphor metabolizing Pseudomonas putida strain NCIMB 10007”. AMB Express. 1: #13—13. PMID 21906366.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Camphor+1,2-monooxygenase на US National Library of Medicine Medical Subject Headings (MeSH)
