Hijaluronan sintaza
| Hijaluronan sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.4.1.212 | ||||||||
| CAS broj | 39346-43-5 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Hijaluronan sintaza (EC 2.4.1.212, spHAS, seHAS) je enzim sa sistematskim imenom Naizmenični UDP-alfa-N-acetil-D-glukozamin:beta-D-glukuronazil-(1->3)-(nascent hijaluronan) 4-N-acetil-beta-D-glukozaminiltransferaza i UDP-alfa-D-glukuronat:N-acetil-beta-D-glukozaminil-(1->4)-(nascentni hijaluronan) 3-beta-D-glukuronaziltransferaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) UDP-alfa-N-acetil-D-glukozamin + beta-D-glukuronazil-(1->3)-N-acetil-beta-D-glukozaminil-(1->4)-[nascentni hijaluronan] UDP + N-acetil-beta-D-glukozaminil-(1->4)-beta-D-glukuronazil-(1->3)-N-acetil-beta-D-glukozaminil-(1->4)-[nascentni hijaluronan]
- (2) UDP-alfa-D-glukuronat + N-acetil-beta-D-glukozaminil-(1->4)-beta-D-glukuronazil-(1->3)-[nascentni hijaluronan] UDP + beta-D-glukuronazil-(1->3)-N-acetil-beta-D-glukozaminil-(1->4)-beta-D-glukuronazil-(1->3)-[nascentni hijaluronan]
Za dejstvo enzima iz Streptococcus grupe A i grupe C je neophodan Mg2+.
Reference[уреди | уреди извор]
- ^ DeAngelis, P.L., Papaconstantinou, J. and Weigel, P.H. (1993). „Molecular cloning, identification and sequence of the hyaluronan synthase gene from Group A Streptococcus pyogenes”. J. Biol. Chem. 268: 19181—19184. PMID 8366070.
- ^ Jing, W. & DeAngelis, P.L. (2000). „Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide”. Glycobiology. 10: 883—889. PMID 10988250.
- ^ DeAngelis, P.L. (1999). „Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase”. J. Biol. Chem. 274: 26557—26562. PMID 10473619.
- ^ Tlapak-Simmons, V.L., Baron, C.A. and Weigel, P.H. (2004). „Characterization of the purified hyaluronan synthase from Streptococcus equisimilis”. Biochemistry. 43: 9234—9242. PMID 15248781.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Hyaluronan+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
