Piridoksal 5'-fosfatna sintaza (hidroliza glutamina)

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Piridoksal 5'-fosfatna sintaza (hidroliza glutamina)
Identifikatori
EC broj 4.3.3.6
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Piridoksal 5'-fosfatna sintaza (hidroliza glutamina) (EC 4.3.3.6, PdxST) je enzim sa sistematskim imenom D-riboza 5-fosfat,D-gliceraldehid 3-fosfat piridoksal 5'-fosfat-lijaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + L-glutamin \rightleftharpoons piridoksal 5'-fosfat + L-glutamat + 3H2O + fosfat (sveukupna reakcija)
(1a) L-glutamin + H2O \rightleftharpoons L-glutamat + NH3
(1b) D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + NH3 \rightleftharpoons piridoksal 5'-fosfat + 4H2O + fosfat

Amonijak obezbeđuje glutaminazna podjedinica.

Reference[уреди]

  1. Burns, K.E., Xiang, Y., Kinsland, C.L., McLafferty, F.W. and Begley, T.P. (2005). „Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway“. J. Am. Chem. Soc. 127: 3682-3683. PMID 15771487. 
  2. Raschle, T., Amrhein, N. and Fitzpatrick, T.B. (2005). „On the two components of pyridoxal 5′-phosphate synthase from Bacillus subtilis“. J. Biol. Chem. 280: 32291-32300. PMID 16030023. 
  3. Strohmeier, M., Raschle, T., Mazurkiewicz, J., Rippe, K., Sinning, I., Fitzpatrick, T.B. and Tews, I. (2006). „Structure of a bacterial pyridoxal 5′-phosphate synthase complex“. Proc. Natl. Acad. Sci. USA 103: 19284-19289. PMID 17159152. 
  4. Raschle, T., Arigoni, D., Brunisholz, R., Rechsteiner, H., Amrhein, N. and Fitzpatrick, T.B. (2007). „Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate“. J. Biol. Chem. 282: 6098-6105. PMID 17189272. 
  5. Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). „Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate“. Angew. Chem. Int. Ed. Engl. 47: 2102-2105. PMID 18260082. 
  6. Hanes, J.W., Burns, K.E., Hilmey, D.G., Chatterjee, A., Dorrestein, P.C. and Begley, T.P. (2008). „Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate“. J. Am. Chem. Soc. 130: 3043-3052. PMID 18271580. 
  7. Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). „13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase“. Nat. Chem. Biol. 4: 425-430. PMID 18516049. 
  8. Wallner, S., Neuwirth, M., Flicker, K., Tews, I. and Macheroux, P. (2009). „Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis“. Biochemistry 48: 1928-1935. PMID 19152323. 

Literatura[уреди]

Spoljašnje vezе[уреди]