WRN (gen)

Из Википедије, слободне енциклопедије
Vernerov sindrom, RecQ helikaza
Struktura DNA- i protein- vezujućeg motiva Vernerovog proteina. PDB prikaz baziran na 2axl.
Dostupne strukture
2AXL, 2DGZ, 2E1E, 2E1F, 2FBT, 2FBV, 2FBX, 2FBY, 2FC0, 3AAF
Identifikatori
Simboli WRN; RECQ3; RECQL2; RECQL3
Vanjski ID OMIM604611 MGI109635 HomoloGene6659 GeneCards: WRN Gene
EC broj 3.6.4.12
Pregled RNK izražavanja
PBB GE WRN 205667 at tn.png
podaci
Ortolozi
Vrsta Čovek Miš
Entrez 7486 22427
Ensembl ENSG00000165392 ENSMUSG00000031583
UniProt Q14191 O09053
RefSeq (mRNA) NM_000553.4 NM_001122822.1
RefSeq (protein) NP_000544.2 NP_001116294.1
Lokacija (UCSC) Chr 8:
30.89 - 31.03 Mb
Chr 8:
34.34 - 34.5 Mb
PubMed pretraga [1] [2]

WRN (Vernerov sindrom) je ljudski gen koji korira Vernerov protein. On je helikaza. Helikaze su enzimi koji generalno odvijaju i razdvajaju lance dvolančane DNK. Te aktivnosti su neophodne pre kopiranja DNK u pripremi za ćelijsku deobu (DNK). Helikaze su takođe kritične u procesu transkripcije. Vernerov protein ima kritičnu ulogu u popravci DNK. Ovaj protein pomaže u održavanju strukture i integriteta DNK.

WRN gen se nalazi na kratkoj (p) ruci hromozoma 8 između pozicija 12 i 11.2, od baznog para 31,010,319 do baznog para 31,150,818.

Interakcije[уреди]

ATP-zavisna helikaza Vernerovog sindroma formira interakcije sa Ku70,[1][2] PCNA,[3][4] DNA-PKcs,[5][6] P53,[7][8] Ku80,[1][2] endonukleaza 1 specifična za Flap strukturu,[9][10] WRNIP1,[11] Bloom sindrom protein[12] i TERF2.[13]

Reference[уреди]

  1. 1,0 1,1 Karmakar, Parimal; Snowden Carey M, Ramsden Dale A, Bohr Vilhelm A (2002). „Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus”. Nucleic Acids Res. England. 30 (16): 3583—91. doi:10.1093/nar/gkf482. PMC 134248Слободан приступ. PMID 12177300. 
  2. 2,0 2,1 Li, B; Comai L (2000). „Functional interaction between Ku and the werner syndrome protein in DNA end processing”. J. Biol. Chem. UNITED STATES. 275 (37): 28349—52. doi:10.1074/jbc.C000289200. ISSN 0021-9258. PMID 10880505. 
  3. Rodríguez-López, Ana M; Jackson Dean A, Nehlin Jan O, Iborra Francisco, Warren Anna V, Cox Lynne S (2003). „Characterisation of the interaction between WRN, the helicase/exonuclease defective in progeroid Werner's syndrome, and an essential replication factor, PCNA”. Mech. Ageing Dev. Ireland. 124 (2): 167—74. doi:10.1016/S0047-6374(02)00131-8. ISSN 0047-6374. PMID 12633936. 
  4. Huang, S; Beresten S, Li B, Oshima J, Ellis N A, Campisi J (2000). „Characterization of the human and mouse WRN 3'-->5' exonuclease”. Nucleic Acids Res. ENGLAND. 28 (12): 2396—405. doi:10.1093/nar/28.12.2396. PMC 102739Слободан приступ. PMID 10871373. 
  5. Kim, S T; Lim D S, Canman C E, Kastan M B (1999). „Substrate specificities and identification of putative substrates of ATM kinase family members”. J. Biol. Chem. UNITED STATES. 274 (53): 37538—43. doi:10.1074/jbc.274.53.37538. ISSN 0021-9258. PMID 10608806. 
  6. Karmakar, Parimal; Piotrowski Jason, Brosh Robert M, Sommers Joshua A, Miller Susan P Lees, Cheng Wen-Hsing, Snowden Carey M, Ramsden Dale A, Bohr Vilhelm A (2002). „Werner protein is a target of DNA-dependent protein kinase in vivo and in vitro, and its catalytic activities are regulated by phosphorylation”. J. Biol. Chem. United States. 277 (21): 18291—302. doi:10.1074/jbc.M111523200. ISSN 0021-9258. PMID 11889123. 
  7. Yang, Qin; Zhang Ran, Wang Xin Wei, Spillare Elisa A, Linke Steven P, Subramanian Deepa, Griffith Jack D, Li Ji Liang, Hickson Ian D, Shen Jiang Cheng, Loeb Lawrence A, Mazur Sharlyn J, Appella Ettore, Brosh Robert M, Karmakar Parimal, Bohr Vilhelm A, Harris Curtis C (2002). „The processing of Holliday junctions by BLM and WRN helicases is regulated by p53”. J. Biol. Chem. United States. 277 (35): 31980—7. doi:10.1074/jbc.M204111200. ISSN 0021-9258. PMID 12080066. 
  8. Brosh, R M; Karmakar P, Sommers J A, Yang Q, Wang X W, Spillare E A, Harris C C, Bohr V A (2001). „p53 Modulates the exonuclease activity of Werner syndrome protein”. J. Biol. Chem. United States. 276 (37): 35093—102. doi:10.1074/jbc.M103332200. ISSN 0021-9258. PMID 11427532. 
  9. Sharma, Sudha; Sommers Joshua A, Wu Leonard, Bohr Vilhelm A, Hickson Ian D, Brosh Robert M (2004). „Stimulation of flap endonuclease-1 by the Bloom's syndrome protein”. J. Biol. Chem. United States. 279 (11): 9847—56. doi:10.1074/jbc.M309898200. ISSN 0021-9258. PMID 14688284. 
  10. Brosh, R M; von Kobbe C, Sommers J A, Karmakar P, Opresko P L, Piotrowski J, Dianova I, Dianov G L, Bohr V A (2001). „Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity”. EMBO J. England. 20 (20): 5791—801. doi:10.1093/emboj/20.20.5791. ISSN 0261-4189. PMC 125684Слободан приступ. PMID 11598021. 
  11. Kawabe Yi, Yi; Branzei D, Hayashi T, Suzuki H, Masuko T, Onoda F, Heo S J, Ikeda H, Shimamoto A, Furuichi Y, Seki M, Enomoto T (2001). „A novel protein interacts with the Werner's syndrome gene product physically and functionally”. J. Biol. Chem. United States. 276 (23): 20364—9. doi:10.1074/jbc.C100035200. ISSN 0021-9258. PMID 11301316. 
  12. von Kobbe, Cayetano; Karmakar Parimal, Dawut Lale, Opresko Patricia, Zeng Xianmin, Brosh Robert M, Hickson Ian D, Bohr Vilhelm A (2002). „Colocalization, physical, and functional interaction between Werner and Bloom syndrome proteins”. J. Biol. Chem. United States. 277 (24): 22035—44. doi:10.1074/jbc.M200914200. ISSN 0021-9258. PMID 11919194. 
  13. Opresko, Patricia L; von Kobbe Cayetano, Laine Jean-Philippe, Harrigan Jeanine, Hickson Ian D, Bohr Vilhelm A (2002). „Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases”. J. Biol. Chem. United States. 277 (43): 41110—9. doi:10.1074/jbc.M205396200. ISSN 0021-9258. PMID 12181313. 

Literatura[уреди]

Spoljašnje veze[уреди]