Dezoksihipuzin sintaza
| Dezoksihipuzin sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.5.1.46 | ||||||||
| CAS broj | 127069-31-2 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Dezoksihipuzin sintaza (EC 2.5.1.46, spermidin:eIF5A-lizin 4-aminobutiltransferaza (formira propan-1,3-diamin)) je enzim sa sistematskim imenom (eIF5A-prekurzor)-lizin:spermidin 4-aminobutiltransferaza (formira propan-1,3-diamin).[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
- [eIF5A-prekurzor]-lizin + spermidin [eIF5A-prekurzor]-dezoksihipuzin + propan-1,3-diamin (sveukupna reakcija)
- (1a) spermidin + NAD+ dehidrospermidin + NADH
- (1b) dehidrospermidin + [enzim]-lizin N-(4-aminobutiliden)-[enzim]-lizin + propan-1,3-diamin
- (1c) N-(4-aminobutiliden)-[enzim]-lizin + [eIF5A-prekurzor]-lizin N-(4-aminobutiliden)-[eIF5A-prekurzor]-lizin + [enzim]-lizin
- (1d) N-(4-aminobutiliden)-[eIF5A-prekurzor]-lizin + NADH + H+ [eIF5A-prekurzor]-dezoksihipuzin + NAD+
Eukariotski inicijacioni faktor eIF5A sadrži hipuzinski ostatak koji je esencijalan za aktivnost. Ovaj enzim katalizuje prvu reakciju formiranja hipuzina.
Reference[уреди | уреди извор]
- ^ Wolff, E.C., Park, M.H. and Folk, J.E. (1990). „Cleavage of spermidine as the first step in deoxyhypusine synthesis. The role of NAD+”. J. Biol. Chem. 265: 4793—4799. PMID 1690726.
- ^ Wolff, E.C., Folk, J.E. and Park, M.H. (1997). „Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase”. J. Biol. Chem. 272: 15865—15871. PMID 9188485.
- ^ Chen, K.Y. & Liu, A.Y.C. (1997). „Biochemistry and function of hypusine formation on eukaryotic initiation factor 5A”. Biol. Signals. 6: 105—109. PMID 9285092.
- ^ Ober, D. & Hartmann, T. (1999). „Deoxyhypusine synthase from tobacco. cDNA isolation, characterization, and bacterial expression of an enzyme with extended substrate specificity”. J. Biol. Chem. 274: 32040—32047. PMID 10542236.
- ^ Ober, D. & Hartmann, T. (1999). „Homospermidine synthase, the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis, evolved from deoxyhypusine synthase”. Proc. Natl. Acad. Sci. USA. 96: 14777—14782. PMID 10611289.
- ^ Wolff, E.C. & Park, M.H. (1999). „Identification of lysine350 of yeast deoxyhypusine synthase as the site of enzyme intermediate formation”. Yeast. 15: 43—50. PMID 10028184.
- ^ Wolff, E.C., Wolff, J. and Park, M.H. (2000). „Deoxyhypusine synthase generates and uses bound NADH in a transient hydride transfer mechanism”. J. Biol. Chem. 275: 9170—9177. PMID 10734052.
- ^ Joe, Y.A., Wolff, E.C. and Park, M.H. (1995). „Cloning and expression of human deoxyhypusine synthase cDNA: structure-function studies with the recombinant enzyme and mutant proteins”. J. Biol. Chem. 270: 22386—22392. PMID 7673224.
- ^ Tao, Y. & Chen, K.Y. (1995). „Molecular cloning and functional expression of Neurospora deoxyhypusine synthase cDNA and identification of yeast deoxyhypusine synthase cDNA”. J. Biol. Chem. 270: 23984—23987. PMID 7592594.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- Deoxyhypusine+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
