RRNK mala podjedinica pseudouridinske metiltransferaze Nep1
| rRNK mala podjedinica pseudouridinske metiltransferaze Nep1 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.260 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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rRNK mala podjedinica pseudouridinske metiltransferaze Nep1 (EC 2.1.1.260, Nep1, nukleolarni esencijalni protein 1) je enzim sa sistematskim imenom S-adenozil-L-metionin:18S rRNK (pseudouridin1191-N1)-metiltransferaza.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- S-adenozil-L-metionin + pseudouridin1191 u kvašćanoj 18S rRNK S-adenozil-L-homocistein + N1-metilpseudouridin1191 u kvašćanoj 18S rRNK
Ovaj enzim se javlja kod prokariota i eukariota. On specifično prepoznaje pseudouridinske ostatke (Psi) u malim podjedinicama ribozomalne RNK na bazi lokalne RNK strukture.
Reference[уреди | уреди извор]
- ^ Taylor, A.B., Meyer, B., Leal, B.Z., Kötter, P., Schirf, V., Demeler, B., Hart, P.J., Entian, K.-D. and Wöhnert, J. (2008). „The crystal structure of Nep1 reveals an extended SPOUT-class methyltransferase fold and a pre-organized SAM-binding site”. Nucleic Acids Res. 36: 1542—1554. PMID 18208838.
- ^ Wurm, J.P., Meyer, B., Bahr, U., Held, M., Frolow, O., Kötter, P., Engels, J.W., Heckel, A., Karas, M., Entian, K.-D. and Wöhnert, J. (2010). „The ribosome assembly factor Nep1 responsible for Bowen-Conradi syndrome is a pseudouridine-N1-specific methyltransferase”. Nucleic Acids Res. 38: 2387—2398. PMID 20047967.
- ^ Meyer, B., Wurm, J.P., Kötter, P., Leisegang, M.S., Schilling, V., Buchhaupt, M., Held, M., Bahr, U., Karas, M., Heckel, A., Bohnsack, M.T., Wöhnert, J. and Entian, K.-D. (2011). „The Bowen-Conradi syndrome protein Nep1 (Emg1) has a dual role in eukaryotic ribosome biogenesis, as an essential assembly factor and in the methylation of Ψ1191 in yeast 18S rRNA”. Nucleic Acids Res. 39: 1526—1537. PMID 20972225.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- RRNA+small+subunit+pseudouridine+methyltransferase+Nep1 на US National Library of Medicine Medical Subject Headings (MeSH)
