Matricom potpomognuta laserska desorpcija/jonizacija — разлика између измена
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Matricom potpomognuta laserska desorpcija/jonizacija (MALDI) is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers, dendrimers and other macromolecules), which tend to be fragile and fragment when ionized by more conventional ionization methods. It is similar in character to electrospray ionization (ESI) in that both techniques are relatively soft ways of obtaining ions of large molecules in the gas phase, though MALDI produces far fewer multiply charged ions.
MALDI methodology is a three-step process. First, the sample is mixed with a suitable matrix material and applied to a metal plate. Second, a pulsed laser irradiates the sample, triggering ablation and desorption of the sample and matrix material. Finally, the analyte molecules are ionized by being protonated or deprotonated in the hot plume of ablated gases, and can then be accelerated into whichever mass spectrometer is used to analyse them.[1]
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Reference
- ^ Karas, Michael; Krüger, Ralf (2003). „Ion Formation in MALDI: The Cluster Ionization Mechanism”. Chemical Reviews. 103 (2): 427—440. ISSN 0009-2665. doi:10.1021/cr010376a.
Literatura
- Hillenkamp, F.; Karas, M.; Beavis, R. C.; Chait, B. T. (1991). „Matrix-assisted laser desorption/ionization mass spectrometry of biopolymers”. Analytical Chemistry. 63 (24): 1193A—1203A. PMID 1789447. doi:10.1021/ac00024a002.
- Ragoussis, J.; Elvidge, G. P.; Kaur, K.; Colella, S. (2006). „Matrix-Assisted Laser Desorption/Ionisation, Time-of-Flight Mass Spectrometry in Genomics Research”. PLoS Genetics. 2 (7): e100. PMC 1523240
. PMID 16895448. doi:10.1371/journal.pgen.0020100. - Hardouin, J. (2007). „Protein sequence information by matrix-assisted laser desorption/ionization in-source decay mass spectrometry”. Mass Spectrometry Reviews. 26 (5): 672—82. PMID 17492750. doi:10.1002/mas.20142.
- Peter-Katalinic, J.; Hillenkamp, F. (2007). MALDI MS: A Practical Guide to Instrumentation, Methods and Applications. Wiley-VCH. ISBN 3-527-31440-7.
- Schrepp, W.; Pasch, H. (2003). MALDI-TOF Mass Spectrometry of Synthetic Polymers. Springer-Verlag. ISBN 3-540-44259-6.
Spoljašnje veze
- Primer on Matrix-Assisted Laser Desorption Ionization (MALDI) National High Magnetic Field Laboratory