Holest-4-en-3-on 26-monooksigenaza
| Holest-4-en-3-on 26-monooksigenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.141 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Holest-4-en-3-on 26-monooksigenaza (EC 1.14.13.141, CYP125, CYP125A1, holest-4-en-3-on 27-monooksigenaza) je enzim sa sistematskim imenom holest-4-en-3-on,NADH:kiseonik oksidoreduktaza (26-hidroksilacija).[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- holest-4-en-3-on + NADH + H+ + O2 26-hidroksiholest-4-en-3-on + NAD+ + H2O
Ovaj hem tiolatni (P450) enzim, prisutan u nekoliko bakterijskih patogena, učestvuje u degradaciji domaćinovog holesterola. On katalizuje hidroksilaciju na C-26, čemu sledi oksidacija alkohola do karboksilne kiseline putem aldehidnog intermedijera.
Reference[уреди | уреди извор]
- ^ Rosloniec, K.Z., Wilbrink, M.H., Capyk, J.K., Mohn, W.W., Ostendorf, M., van der Geize, R., Dijkhuizen, L. and Eltis, L.D. (2009). „Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1”. Mol. Microbiol. 74: 1031—1043. PMID 19843222.
- ^ McLean, K.J., Lafite, P., Levy, C., Cheesman, M.R., Mast, N., Pikuleva, I.A., Leys, D. and Munro, A.W. (2009). „The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection”. J. Biol. Chem. 284: 35524—35533. PMID 19846552.
- ^ Capyk, J.K., Kalscheuer, R., Stewart, G.R., Liu, J., Kwon, H., Zhao, R., Okamoto, S., Jacobs, W.R., Jr., Eltis, L.D. and Mohn, W.W. (2009). „Mycobacterial cytochrome P450 125 (cyp125) catalyzes the terminal hydroxylation of C27 steroids”. J. Biol. Chem. 284: 35534—35542. PMID 19846551.
- ^ Ouellet, H., Guan, S., Johnston, J.B., Chow, E.D., Kells, P.M., Burlingame, A.L., Cox, J.S., Podust, L.M. and de Montellano, P.R. (2010). „Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one”. Mol. Microbiol. 77: 730—742. PMID 20545858.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- Cholest-4-en-3-one+26-monooxygenase на US National Library of Medicine Medical Subject Headings (MeSH)
