Извор странице Dizajn proteina

'''Dizajn proteina''' je proces formiranja novih [[protein]]a, bilo -{''de novo''}- ili putem pravljenja proračunatih varijacija poznatih struktura. Upotreba tehnika [[racionalni dizajn|racionalnog dizajna]] je glavni aspekt [[proteinsko inženjerstvo|proteinskog inženjerstva]].

Dizajn računarskih modela proteina, i [[Секундарна структура протеина|sekundarne strukturne]] modifikacije realnih proteina su počeli sredinom [[1990e|1990-tih]]. ''De novo'' dizajn realnih proteina je postao moguć uskoro nakon toga, i u [[21. vek]]u je to postalo produktivno naučno polje.

== Primeri dezajna proteina ==
U ranom [[21. vek]]u je počeo razvoj malih proteina sa realnim biološkim funkcijama, uključujući [[Хиралност молекула|stereoselektivnu]] katalizu,<ref>{{cite journal |author1=Saghatelian, Alan |author2=Yokobayashi, Yohei |author3=Soltani, Kathy |author4=Ghadiri, M. Reza  |lastauthoramp=yes |year=2001 |title=A chiroselective peptide replicator |journal= Nature |volume=409 |issue=6822 |pages=797–801 |doi=10.1038/35057238 |pmid=11236988 |url=http://www.nature.com/nature/journal/v409/n6822/abs/409797a0.html}}</ref> detekciju jona,<ref>{{cite journal |author1=Nagai, Takeharu |author2=Sawano, Asako |author3=Park, Eun Sun |author4=Miyawaki, Atsushi  |lastauthoramp=yes |year=2001 |title=Circularly permuted green fluorescent proteins engineered to sense Ca<sup>2+</sup> |journal=Proceedings of the National Academy of Sciences |pmid=11248055 |volume=98 |issue=6 |pmc=30630 |pages=3197–3202 |doi=10.1073/pnas.051636098 |bibcode = 2001PNAS...98.3197N }}</ref> i [[virus|antiviralno]] dejstvo.<ref>{{cite journal |author1=Root, Michael J. |author2=Kay, Michael S. |author3=Kim, Peter S. |year=2001 |title=Protein design of an HIV-1 entry inhibitor |journal = Science |volume=291 |issue=5505 |pages=884–888 |doi=10.1126/science.1057453 |url=http://www.sciencemag.org/content/291/5505/884.abstract|bibcode = 2001Sci...291..884R }}</ref> Koristeći računarske metode, protein sa novim strukturnim motivom je dizajniran, koji se naziva -{[[Top7]]}-,<ref>{{cite journal |author1=Kuhlman, Brian |author2=Dantas, Gautam |author3=Ireton, Gregory C. |author4=Varani, Gabriele |author5=Stoddard, Barry L. |author6=Baker, David  |lastauthoramp=yes |year=2003 |title=Design of a Novel Globular Protein Fold with Atomic-Level Accuracy |journal=Science |volume=302 |issue=5649 |pages=1364–1368 |doi=10.1126/science.1089427 |pmid=14631033 |bibcode=2003Sci...302.1364K}}</ref> kao i senzori za veštačke molekule.<ref>{{cite journal |author1=Looger, Loren L. |author2=Dwyer, Mary A. |author3=Smith, James J. |author4=Hellinga, Homme W.  |lastauthoramp=yes |year=2003 |title=Computational design of receptor and sensor proteins with novel functions |journal=Nature |volume=423 |issue=6936 |pages=185–190 |doi=10.1038/nature01556 |pmid=12736688|bibcode = 2003Natur.423..185L }}</ref> Dizajn [[fuzioni protein|fuzionih proteina]] je proizveo [[rilonacept]], lek koji je [[FDA]] odobrila za lečenje kriopirin vezanog periodičnog sindroma. Uz pomoć računarskog redizajna bilo je moguće promeniti specifičnot [[kofaktor]]a -{''Candida boidinii''}- ksilozne reduktaze od [[Nikotinamid adenin dinukleotid fosfat|NADPH]] u [[Nikotinamid adenin dinukleotid|NADH]].<ref name="khoury">{{cite journal |title=Computational design of Candida boidinii xylose reductase for altered cofactor specificity |journal=Protein Science |volume=18 |issue=10 |pages=2125–38 |year=2009 |doi=10.1002/pro.227 |pmc=2786976 |pmid=19693930 |author1=Khoury, GA |author2=Fazelinia, H |author3=Chin, JW |author4=Pantazes, RJ |author5=Cirino, PC |author6=Maranas, CD }}</ref>

== Reference ==
{{reflist|2}}

== Literatura ==
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}-{{Литература крај}}

== Vidi još ==
* [[Proteinsko inženjerstvo]]
* [[Predviđanje strukture proteina]]
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{{Biomolekularne strukture-lat}}

{{normativna kontrola}}

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