NT5E

5'-nucleotidase ecto
Identifikatori
Alijasi
Spoljašnji ID	GeneCards: [1]
Ortolozi
Vrste	Čovek	Miš
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a
Wikidata
View/Edit Human

5'-nukleotidaza (5'-NT), poznata i kao ekto-5'-nukleotidaza ili CD73 (klaster diferencijacije 73), je enzim koji je kod ljudi kodiran NT5E genom. CD73 konvertuje AMP u adenozin.

Funkcija

Ekto-5-prajm-nukleotidaza (5-prajm-ribonukleotidna fosfohidrolaza; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Vidi još

• Klasteri diferencijacije

Literatura

• Resta R, Thompson LF (1997). „T cell signalling through CD73.”. Cell. Signal. 9 (2): 131—9. PMID 9113412. doi:10.1016/S0898-6568(96)00132-5. 
• Kirchhoff C, Hale G (1997). „Cell-to-cell transfer of glycosylphosphatidylinositol-anchored membrane proteins during sperm maturation.”. Mol. Hum. Reprod. 2 (3): 177—84. PMID 9238677. doi:10.1093/molehr/2.3.177. 
• Resta R, Yamashita Y, Thompson LF (1998). „Ecto-enzyme and signaling functions of lymphocyte CD73.”. Immunol. Rev. 161: 95—109. PMID 9553767. doi:10.1111/j.1600-065X.1998.tb01574.x. 
• Rosi F, Carlucci F, Marinello E, Tabucchi A (2002). „Ecto-5'-nucleotidase in B-cell chronic lymphocytic leukemia.”. Biomed. Pharmacother. 56 (2): 100—4. PMID 12000134. doi:10.1016/S0753-3322(01)00072-5. 
• Babiychuk EB, Draeger A (2006). „Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?”. Biochem. Soc. Trans. 34 (Pt 3): 374—6. PMID 16709165. doi:10.1042/BST0340374. 
• Stefanovic V, Mandel P, Rosenberg A (1976). „Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells.”. J. Biol. Chem. 251 (13): 3900—5. PMID 819433. 
• Thomson LF, Ruedi JM, Glass A, et al. (1990). „Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).”. Tissue Antigens. 35 (1): 9—19. PMID 2137649. doi:10.1111/j.1399-0039.1990.tb01750.x. 
• Klemens MR, Sherman WR, Holmberg NJ, et al. (1990). „Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.”. Biochem. Biophys. Res. Commun. 172 (3): 1371—7. PMID 2173922. doi:10.1016/0006-291X(90)91601-N. 
• Boyle JM, Hey Y, Guerts van Kessel A, Fox M (1989). „Assignment of ecto-5'-nucleotidase to human chromosome 6.”. Hum. Genet. 81 (1): 88—92. PMID 2848759. doi:10.1007/BF00283737. 
• Vlahović P, Stefanović V (1995). „Effect of dopamine on ecto-5'-nucleotidase expression in human glomerular mesangial cells.”. Archives internationales de physiologie, de biochimie et de biophysique. 102 (3): 171—3. PMID 8000038. 
• Hansen KR, Resta R, Webb CF, Thompson LF (1996). „Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene.”. Gene. 167 (1-2): 307—12. PMID 8566797. doi:10.1016/0378-1119(95)00574-9. 
• Airas L, Jalkanen S (1996). „CD73 mediates adhesion of B cells to follicular dendritic cells.”. Blood. 88 (5): 1755—64. PMID 8781432. 
• Airas L, Niemelä J, Salmi M, et al. (1997). „Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells.”. J. Cell Biol. 136 (2): 421—31. PMC 2134816 . PMID 9015312. doi:10.1083/jcb.136.2.421. 
• Strohmeier GR, Lencer WI, Patapoff TW, et al. (1997). „Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia.”. J. Clin. Invest. 99 (11): 2588—601. PMC 508104 . PMID 9169488. doi:10.1172/JCI119447. 
• Aumüller G, Renneberg H, Schiemann PJ, et al. (1998). „The role of apocrine released proteins in the post-testicular regulation of human sperm function.”. Adv. Exp. Med. Biol. 424: 193—219. PMID 9361795. doi:10.1007/978-1-4615-5913-9_39. 
• Rosi F, Agostinho AB, Carlucci F, et al. (1998). „Behaviour of human lymphocytic isoenzymes of 5'-nucleotidase.”. Life Sci. 62 (25): 2257—66. PMID 9651114. doi:10.1016/S0024-3205(98)00206-9. 

Spoljašnje veze

• NT5E+protein,+human на US National Library of Medicine Medical Subject Headings (MeSH)