B3GAT2

B3GAT2
Available structures
PDB	Pretraga ortologa: PDBe RCSB
List of PDB id codes
	2D0J
Identifikatori
Alijasi	B3GAT2
Spoljašnji ID	OMIM: 607497 MGI: 2389490 HomoloGene: 50574 GeneCards: B3GAT2
Genska lokacija (miš)
Hr.	Chromosome 1 (mouse)
Kraj	23,888,239 bp
Genska ontologija
Molecular function	• transferase activity; • metal ion binding; • galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity;
Cellular component	• саставни део мембране; • Голџијев апарат; • мембрана; • Golgi membrane;
Biological process	• glycosaminoglycan metabolic process; • GO:0033578, GO:0033577, GO:0033575, GO:0033576 protein glycosylation; • chondroitin sulfate proteoglycan biosynthetic process; • chondroitin sulfate metabolic process; • Метаболизам угљених хидрата;
	Sources:Amigo / QuickGO
Ortolozi
	135152
	280645
	ENSG00000112309
	ENSMUSG00000026156
	Q9NPZ5
	P59270
	NM_080742
	NM_172124
	NP_542780
	NP_742122
	Wikidata
View/Edit Human	View/Edit Mouse

Galaktozilgalaktozilksilozilprotein 3-beta-glukuronoziltransferaza 2 je enzim koji je kod ljudi kodiran genom B3GAT2.^[4]^[5]

Produkt ovog gena je transmembranski protein koji pripada porodici glukuroniltransferaza i katalizuje prenos beta-1,3 povezane glukuronske kiseline na terminalnu galaktozu u različitim glikoproteinima ili glikolipidima koji sadrže Gal-beta-1-4GlcNAc ili Gal-beta-1-3GlcNAc ostatak. Kodirani protein uključen je u sintezu ugljenohidratnog epitopa ljudskog prirodnog ubice-1 (HNK-1), sulfatiranog trisaharida uključenog u ćelijsku migraciju i adheziju u nervnom sistemu.^[5]

Upotreba antitela HNK-1 za istraživanje neuralnog grebena[уреди | уреди извор]

Antitela podignuta protiv epitopa HNK-1 odigrala su veliku ulogu u studijama nervnog grebena, posebno kod ptičijeg embriona.^[6] Prvo antitelo razvijeno protiv ovog epitopa bilo je NC-1,^[7] koje je omogućilo mnogo lakše analize puteva migracije nervnog grebena. Kod ptica, a posebno kod drugih kičmenjaka, rezultate HNK-1 bojenja treba tumačiti sa oprezom jer epitop nije jedinstven za neuralni greben.

Reference[уреди | уреди извор]

^ ^а ^б ^в GRCm38: Ensembl release 89: ENSMUSG00000026156 - Ensembl, May 2017
^ „Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ „Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Imiya K, Ishizaki T, Seiki T, Saito F, Inazawa J, Oka S, Kawasaki T (октобар 2002). „cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope”. Gene. 296 (1–2): 29—36. PMID 12383500. doi:10.1016/S0378-1119(02)00840-5.
^ ^а ^б „Entrez Gene: B3GAT2 beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)”.
^ Bronner-Fraser M (мај 1986). „Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1”. Dev. Biol. 115 (1): 44—55. PMID 3516760. doi:10.1016/0012-1606(86)90226-5.
^ Vincent M, Thiery JP (јун 1984). „A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system”. Dev. Biol. 103 (2): 468—81. PMID 6202575. doi:10.1016/0012-1606(84)90334-8.

Literatura[уреди | уреди извор]

Nagase T, Kikuno R, Ohara O (2002). „Prediction of the coding sequences of unidentified human genes. XXII. The complete sequences of 50 new cDNA clones which code for large proteins.”. DNA Res. 8 (6): 319—27. PMID 11853319. doi:10.1093/dnares/8.6.319 .
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). „Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.”. Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899—903. Bibcode:2002PNAS...9916899M. PMC 139241 . PMID 12477932. doi:10.1073/pnas.242603899 .
Marcos I, Galán JJ, Borrego S, Antiñolo G (2003). „Cloning, characterization, and chromosome mapping of the human GlcAT-S gene.”. J. Hum. Genet. 47 (12): 677—80. PMID 12522689. doi:10.1007/s100380200103 .
Mungall AJ, Palmer SA, Sims SK, et al. (2003). „The DNA sequence and analysis of human chromosome 6.”. Nature. 425 (6960): 805—11. Bibcode:2003Natur.425..805M. PMID 14574404. doi:10.1038/nature02055 .
Ota T, Suzuki Y, Nishikawa T, et al. (2004). „Complete sequencing and characterization of 21,243 full-length human cDNAs.”. Nat. Genet. 36 (1): 40—5. PMID 14702039. doi:10.1038/ng1285 .
Kakuda S, Oka S, Kawasaki T (2004). „Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli.”. Protein Expr. Purif. 35 (1): 111—9. PMID 15039073. doi:10.1016/j.pep.2003.12.021.
Kakuda S, Sato Y, Tonoyama Y, et al. (2005). „Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate.”. Glycobiology. 15 (2): 203—10. PMID 15470230. doi:10.1093/glycob/cwi001 .
Kimura K, Wakamatsu A, Suzuki Y, et al. (2006). „Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes.”. Genome Res. 16 (1): 55—65. PMC 1356129 . PMID 16344560. doi:10.1101/gr.4039406.
Shiba T, Kakuda S, Ishiguro M, et al. (2006). „Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope.”. Proteins. 65 (2): 499—508. PMID 16897771. S2CID 31290955. doi:10.1002/prot.21118.

Spoljašnje veze[уреди | уреди извор]

Human B3GAT2 genome location and B3GAT2 gene details page in the UCSC Genome Browser.
PDBe-KB provides an overview of all the structure information available in the PDB for Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (B3GAT2)

[refGRCm38Ensembl-1] а ^б ^в GRCm38: Ensembl release 89: ENSMUSG00000026156 - Ensembl, May 2017

[2] „Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.

[3] „Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid12383500-4] Imiya K, Ishizaki T, Seiki T, Saito F, Inazawa J, Oka S, Kawasaki T (октобар 2002). „cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope”. Gene. 296 (1–2): 29—36. PMID 12383500. doi:10.1016/S0378-1119(02)00840-5.

[entrez-5] а ^б „Entrez Gene: B3GAT2 beta-1,3-glucuronyltransferase 2 (glucuronosyltransferase S)”.

[6] Bronner-Fraser M (мај 1986). „Analysis of the early stages of trunk neural crest migration in avian embryos using monoclonal antibody HNK-1”. Dev. Biol. 115 (1): 44—55. PMID 3516760. doi:10.1016/0012-1606(86)90226-5.

[7] Vincent M, Thiery JP (јун 1984). „A cell surface marker for neural crest and placodal cells: further evolution in peripheral and central nervous system”. Dev. Biol. 103 (2): 468—81. PMID 6202575. doi:10.1016/0012-1606(84)90334-8.

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