Kortizon b-reduktaza
| Kortizon b-reduktaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.3.1.3 | ||||||||
| CAS broj | 2604034 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Kortizon b-reduktaza (EC 1.3.1.3, 3-okso-Delta4-steroid 5beta-reduktaza, 5beta-reduktaza, androstendionska 5beta-reduktaza, holestenonska 5beta-reduktaza, kortizonska 5beta-reduktaza, kortizonska beta-reduktaza, kortizonska Delta4-5beta-reduktaza, steroidna 5beta-reduktaza, testosteronska 5beta-reduktaza, Delta4-3-ketosteroidna 5beta-reduktaza, Delta4-5beta-reduktaza, Delta4-hidrogenaza, 4,5beta-dihidrokortizon:NADP+ Delta4-oksidoreduktaza, 3-okso-5beta-steroid:NADP+ Delta4-oksidoreduktaza) je enzim sa sistematskim imenom 5beta-holestan-3-on:NADP+ 4,5-oksidoreduktaza.[1][2][3][4][5][6][7][8][9] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) 5beta-holestan-3-on + NADP+ holest-4-en-3-on + NADPH + H+
- (2) 17,21-dihidroksi-5beta-pregnan-3,11,20-trion + NADP+ kortizon + NADPH + H+
Ovaj ljudski enzim efikasno katalizuje redukciju progesterona, androstendiona, 17alfa-hidroksiprogesterona i testosteron do 5beta-redukovanih metabolita; on takođe deluje na aldosteron, kortikosteron i kortizol, mada u manjoj meri. Intermedijeri žučne kiseline 7alfa,12alfa-dihidroksi-4-holesten-3-on i 7alfa-hidroksi-4-holesten-3-on takođe mogu da budu supstrat.
Reference[уреди | уреди извор]
- ^ Forchielli, E. & Dorfman, R.I. (1956). „Separation of Δ4-5α- and Δ4-5β-hydrogenases from rat liver homogenates”. J. Biol. Chem. 223: 443—448. PMID 13376613.
- ^ Brown-Grant, K., Forchielli, E. and Dorfman, R.I. (1960). „The Δ4-hydrogenases of guinea pig adrenal gland”. J. Biol. Chem. 235: 1317—1320. PMID 13805063.
- ^ Levy, H.R. & Talalay, P. (1957). „Enzymatic introduction of double bonds into steroid ring A”. J. Am. Chem. Soc. 79: 2658—2659.
- ^ Tomkins, G.M. (1957). „The enzymatic reduction of Δ4-3-ketosteroids”. J. Biol. Chem. 225: 13—24. PMID 13416214.
- ^ Sugimoto, Y., Yoshida, M. and Tamaoki, B. (1990). „Purification of 5β-reductase from hepatic cytosol fraction of chicken”. J. Steroid Biochem. 37: 717—724. PMID 2278855.
- ^ Furuebisu, M., Deguchi, S. and Okuda, K. (1987). „Identification of cortisone 5β-reductase as Δ4-3-ketosteroid 5β-reductase”. Biochim. Biophys. Acta. 912: 110—114. PMID 3828348.
- ^ Okuda, A. & Okuda, K. (1984). „Purification and characterization of Δ4-3-ketosteroid 5β-reductase”. J. Biol. Chem. 259: 7519—7524. PMID 6736016.
- ^ Charbonneau, A. & The, V.L. (2001). „Genomic organization of a human 5β-reductase and its pseudogene and substrate selectivity of the expressed enzyme”. Biochim. Biophys. Acta. 1517: 228—235. PMID 11342103.
- ^ Kondo, K.H., Kai, M.H., Setoguchi, Y., Eggertsen, G., Sjöblom, P., Setoguchi, T., Okuda, K.I. and Björkhem, I. (1994). „Cloning and expression of cDNA of human Δ4-3-oxosteroid 5β-reductase and substrate specificity of the expressed enzyme”. Eur. J. Biochem. 219: 357—363. PMID 7508385.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Delta4-3-oxosteroid+5beta-reductase на US National Library of Medicine Medical Subject Headings (MeSH)
