Пиридоксал 5'-фосфатна синтаза (хидролиза глутамина)
| Piridoksal 5'-fosfatna sintaza (hidroliza glutamina) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.3.3.6 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Piridoksal 5'-fosfatna sintaza (hidroliza glutamina) (EC 4.3.3.6, PdxST) je enzim sa sistematskim imenom D-riboza 5-fosfat,D-gliceraldehid 3-fosfat piridoksal 5'-fosfat-lijaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
- D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + L-glutamin piridoksal 5'-fosfat + L-glutamat + 3H2O + fosfat (sveukupna reakcija)
- (1a) L-glutamin + H2O L-glutamat + NH3
- (1b) D-riboza 5-fosfat + D-gliceraldehid 3-fosfat + NH3 piridoksal 5'-fosfat + 4H2O + fosfat
Amonijak obezbeđuje glutaminazna podjedinica.
Reference[уреди | уреди извор]
- ^ Burns, K.E., Xiang, Y., Kinsland, C.L., McLafferty, F.W. and Begley, T.P. (2005). „Reconstitution and biochemical characterization of a new pyridoxal-5′-phosphate biosynthetic pathway”. J. Am. Chem. Soc. 127: 3682—3683. PMID 15771487.
- ^ Raschle, T., Amrhein, N. and Fitzpatrick, T.B. (2005). „On the two components of pyridoxal 5′-phosphate synthase from Bacillus subtilis”. J. Biol. Chem. 280: 32291—32300. PMID 16030023.
- ^ Strohmeier, M., Raschle, T., Mazurkiewicz, J., Rippe, K., Sinning, I., Fitzpatrick, T.B. and Tews, I. (2006). „Structure of a bacterial pyridoxal 5′-phosphate synthase complex”. Proc. Natl. Acad. Sci. USA. 103: 19284—19289. PMID 17159152.
- ^ Raschle, T., Arigoni, D., Brunisholz, R., Rechsteiner, H., Amrhein, N. and Fitzpatrick, T.B. (2007). „Reaction mechanism of pyridoxal 5′-phosphate synthase. Detection of an enzyme-bound chromophoric intermediate”. J. Biol. Chem. 282: 6098—6105. PMID 17189272.
- ^ Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). „Trapping of a chromophoric intermediate in the Pdx1-catalyzed biosynthesis of pyridoxal 5′-phosphate”. Angew. Chem. Int. Ed. Engl. 47: 2102—2105. PMID 18260082.
- ^ Hanes, J.W., Burns, K.E., Hilmey, D.G., Chatterjee, A., Dorrestein, P.C. and Begley, T.P. (2008). „Mechanistic studies on pyridoxal phosphate synthase: the reaction pathway leading to a chromophoric intermediate”. J. Am. Chem. Soc. 130: 3043—3052. PMID 18271580.
- ^ Hanes, J.W., Keresztes, I. and Begley, T.P. (2008). „13C NMR snapshots of the complex reaction coordinate of pyridoxal phosphate synthase”. Nat. Chem. Biol. 4: 425—430. PMID 18516049.
- ^ Wallner, S., Neuwirth, M., Flicker, K., Tews, I. and Macheroux, P. (2009). „Dissection of contributions from invariant amino acids to complex formation and catalysis in the heteromeric pyridoxal 5-phosphate synthase complex from Bacillus subtilis”. Biochemistry. 48: 1928—1935. PMID 19152323.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- Pyridoxal+5'-phosphate+synthase+(glutamine+hydrolyzing) на US National Library of Medicine Medical Subject Headings (MeSH)
