3-izopropilmalat dehidrogenaza
| 3-izopropilmalat dehidrogenaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.85 | ||||||||
| CAS broj | 9030-97-1 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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3-izopropilmalat dehidrogenaza (EC 1.1.1.85, beta-isopropilmalatni enzim, beta-izopropilmalatna dehidrogenaza, treo-Ds-3-izopropilmalatna dehidrogenaza, 3-karboksi-2-hidroksi-4-metilpentanoat:NAD+ oksidoreduktaza) je enzim sa sistematskim imenom (2R,3S)-3-izopropilmalat:NAD+ oksidoreduktaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (2R,3S)-3-izopropilmalat + NAD+ 4-metil-2-oksopentanoat + CO2 + NADH + H+ (sveukupna reakcija)
- (1a) (2R,3S)-3-izopropilmalat + NAD+ (2S)-2-izopropil-3-oksosukcinat + NADH + H+
- (1b) (2S)-2-izopropil-3-oksosukcinat 4-metil-2-oksopentanoat + CO2 (spontano)
Produkt se spontano dekarboksiluje i proizvodi 4-metil-2-oksopentanoat.
Reference[уреди | уреди извор]
- ^ Burns, R.O., Umbarger, H.E. and Gross, S.R. (1963). „The biosynthesis of leucine. III. The conversion of α-hydroxy-β-carboxyisocaproate to α-ketoisocaproate”. Biochemistry. 2: 1053—1053. PMID 14087358.
- ^ Parsons, S.J. & Burns, R.O. (1969). „Purification and properties of β-isopropylmalate dehydrogenase”. J. Biol. Chem. 244: 996—1003. PMID 4889950.
- ^ Németh, A., Svingor, Á., Pócsik, M., Dobó, J., Magyar, C, Szilaaagyi, A., Gál, P. and Závodszky, P. (2000). „Mirror image mutations reveal the significance of an intersubunit ion cluster in the stability of 3-isopropylmalate dehydrogenase”. FEBS Lett. 468: 48—52. PMID 10683439.
- ^ Calvo, J. (1964). „M., Stevens, C. M., Kalyanpur, M. G., and Umbarger, H. E. The absolute configuration of α-hydroxy-β-carboxyisocaproic acid (3-isopropylmalic acid), an intermediate in leucine biosynthesis”. Biochemistry. 3: 2024—2027. PMID 14269331.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- 3-isopropylmalate+dehydrogenase на US National Library of Medicine Medical Subject Headings (MeSH)
