5-epiaristolohen 1,3-dihidroksilaza
| 5-epiaristolohen 1,3-dihidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.119 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
5-epiaristolohen 1,3-dihidroksilaza (EC 1.14.13.119, 5-epi-aristolohenska 1,3-dihidroksilaza, EAH) je enzim sa sistematskim imenom 5-epiaristolohen,NADPH:kiseonik oksidoreduktaza (1- i 3-hidroksilacija).[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 5-epiaristolohen + 2 NADPH + 2 H+ + 2 O2 kapsidiol + 2 NADP+ + 2H2O
Ovaj enzim je hem-tiolatni protein (P-450). Kinetička istraživanja sugerišu da se uglavnom 1beta-hidroksiepiaristolohen prvo formira, i da tome sledi hidroksilacija na C-3.
Reference[уреди | уреди извор]
- ^ Ralston, L., Kwon, S.T., Schoenbeck, M., Ralston, J., Schenk, D.J., Coates, R.M. and Chappell, J. (2001). „Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum)”. Arch. Biochem. Biophys. 393: 222—235. PMID 11556809.
- ^ Takahashi, S., Zhao, Y., O'Maille, P.E., Greenhagen, B.T., Noel, J.P., Coates, R.M. and Chappell, J. (2005). „Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes”. J. Biol. Chem. 280: 3686—3696. PMID 15522862.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- 5-epiaristolochene+1,3-dihydroxylase на US National Library of Medicine Medical Subject Headings (MeSH)
