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Adenozilkobinamid-fosfat guanililtransferaza

С Википедије, слободне енциклопедије
(преусмерено са EC 2.7.7.62)
Adenozilkobinamid-fosfat guanililtransferaza
Identifikatori
EC broj2.7.7.62
CAS broj169592-55-6
Baze podataka
IntEnzIntEnz pregled
BRENDABRENDA pristup
ExPASyNiceZyme pregled
KEGGKEGG pristup
MetaCycmetabolički put
PRIAMprofil
Strukture PBPRCSB PDB PDBe PDBj PDBsum

Adenozilkobinamid-fosfat guanililtransferaza (EC 2.7.7.62, CobU, adenozilkobinamid kinaza/adenozilkobinamid-fosfat guanililtransferaza, AdoCbi kinaza/AdoCbi-fosfat guanililtransferaza) je enzim sa sistematskim imenom GTP:adenozilkobinamid-fosfat guanililtransferaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju

GTP + adenozilkobinamid fosfat difosfat + adenozilkobinamid-GDP

Kod Salmonella typhimurium LT2 pod anaerobnim uslovima, CobU (EC 2.7.7.62 i EC 2.7.1.156), CobT (EC 2.4.2.21), CobC (EC 3.1.3.73) i CobS (EC 2.7.8.26) katalizuju reakcije kojima se formira nukleotidna petlja, čime se konvertuje adenozilkobinamid (AdoCbi) u adenozilkobalamin (AdoCbl).

  1. ^ O'Toole, G.A. & Escalante-Semerena, J.C. (1995). „Purification and characterization of the bifunctional CobU enzyme of Salmonella typhimurium LT2. Evidence for a CobU-GMP intermediate”. J. Biol. Chem. 270: 23560—23569. PMID 7559521. 
  2. ^ Thompson, T.B., Thomas, M.G., Escalante-Semerena, J.C. and Rayment, I. (1998). „Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase from Salmonella typhimurium determined to 2.3 Å resolution”. Biochemistry. 37: 7686—7695. PMID 9601028. 
  3. ^ Thompson, T.B., Thomas, M.G., Escalante-Semerena, J.C. and Rayment, I. (1999). „Three-dimensional structure of adenosylcobinamide kinase/adenosylcobinamide phosphate guanylyltransferase (CobU) complexed with GMP: evidence for a substrate-induced transferase active site”. Biochemistry. 38: 12995—13005. PMID 10529169. 
  4. ^ Thomas, M.G., Thompson, T.B., Rayment, I. and Escalante-Semerena, J.C. (2000). „Analysis of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) enzyme of Salmonella typhimurium LT2. Identification of residue His-46 as the site of guanylylation”. J. Biol. Chem. 275: 27576—27586. PMID 10869342. 
  5. ^ Warren, M.J., Raux, E., Schubert, H.L. and Escalante-Semerena, J.C. (2002). „The biosynthesis of adenosylcobalamin (vitamin B12)”. Nat. Prod. Rep. 19: 390—412. PMID 12195810. 

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