Glicerol-1-fosfat dehidrogenaza (NAD(P)+)
| Glicerol-1-fosfat dehidrogenaza (NAD(P)+) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.1.1.261 | ||||||||
| CAS broj | 204594-18-3 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Glicerol-1-fosfat dehidrogenaza (NAD(P)+) (EC 1.1.1.261, sn-glicerol-1-fosfat:NAD+ oksidoreduktaza, G-1-P dehidrogenaza, Gro1PDH, AraM) je enzim sa sistematskim imenom sn-glicerol-1-fosfat:NAD(P)+ 2-oksidoreduktaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
- sn-glicerol-1-fosfat + NAD(P)+ gliceron fosfat + NAD(P)H + H+
Ovaj enzim se prvenstveno nalazi u Zn2+-zavisnoj formi kod arheja, dok je Ni2+-zavisna forma prisutna kod Gram-pozitivnih bakterija. Zn2+-zavisni metaloenzim je odgovoran za formiranje arhejski-specifičnog sn-glicerol-1-fosfata, u prvom koraku biosinteze polarnih lipida. On je enantiomer sn-glicerol 3-fosfata, forme glicerofosfata prisutnog kod bakterija i eukariota. Drugi enzimi koji učestvuju u biosintezi polarnih lipida kod arheja su EC 2.5.1.41, fosfoglicerol geranilgeraniltransferaza, i EC 2.5.1.42, (geranilgeranilglicerol-fosfat geranilgeraniltransferaza, koji zajedno alkiliraju hidroksi grupe glicerol 1-fosfata i proizvode nezasićenu arhetidinsku kiselinu, na koju deluje EC 2.7.7.67, CDP-arheolna sintaza, da formira CDP-nezasićeni arheol. Krajnji korak ovog sintetičkog puta je adicija L-serina, sa pratećim uklanjanjem CMP-a, što dovodi do produkcije nezasićenog arhetidilserina. Aktivnost ovog enzima stimuliše K+.
Reference[уреди | уреди извор]
- ^ Nishihara, M. & Koga, Y. (1995). „sn-Glycerol-1-phosphate dehydrogenase in Methanobacterium thermoautotrophicum: key enzyme in biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of archaebacteria”. J. Biochem. 117: 933—935. PMID 8586635.
- ^ Nishihara, M. & Koga, Y. (1997). „Purification and properties of sn-glycerol-1-phosphate dehydrogenase from Methanobacterium thermoautotrophicum: characterization of the biosynthetic enzyme for the enantiomeric glycerophosphate backbone of ether polar lipids of Archaea”. J. Biochem. 122: 572—576. PMID 9348086.
- ^ Koga, Y., Kyuragi, T., Nishihara, M. and Sone, N. (1998). „Did archaeal and bacterial cells arise independently from noncellular precursors? A hypothesis stating that the advent of membrane phospholipid with enantiomeric glycerophosphate backbones caused the separation of the two lines of descent”. J. Mol. Evol. 46: 54—63. PMID 9419225.
- ^ Morii, H., Nishihara, M. and Koga, Y. (2000). „CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase in the methanogenic archaeon Methanothermobacter thermoautotrophicus”. J. Biol. Chem. 275: 36568—36574. PMID 10960477.
- ^ Han, J.S. & Ishikawa, K. (2005). „Active site of Zn2+-dependent sn-glycerol-1-phosphate dehydrogenase from Aeropyrum pernix K1”. Archaea. 1: 311—317. PMID 15876564.
- ^ Guldan, H., Sterner, R. and Babinger, P. (2008). „Identification and characterization of a bacterial glycerol-1-phosphate dehydrogenase: Ni(2+)-dependent AraM from Bacillus subtilis”. Biochemistry. 47: 7376—7384. PMID 18558723.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- glycerol-1-phosphate+dehydrogenase на US National Library of Medicine Medical Subject Headings (MeSH)
