Izoflavonoid sintaza
| Izoflavonoid sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.136 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Izoflavonoid sintaza (EC 1.14.13.136, CYT93C, IFS, 2-hidroksiizoflavanonska sintaza (nespecifična)) je enzim sa sistematskim imenom likviritigenin,NADPH:kiseonik oksidoreduktaza (hidroksilacija, aril migracija).[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
- liquiritigenin + O2 + NADPH + H+ 2,7,4'-trihidroksiizoflavanon + H2O + NADP+
Za rad ovog enzim je neophodan citohrom P450. Reakcija obuhvata migraciju 2-fenil grupe flavanon likviritigenina do 3-pozicije izoflavanona.
Reference[уреди | уреди извор]
- ^ Hashim, M.F., Hakamatsuka, T., Ebizuka, Y. and Sankawa, U. (1990). „Reaction mechanism of oxidative rearrangement of flavanone in isoflavone biosynthesis”. FEBS Lett. 271: 219—222. PMID 2226805.
- ^ Sawada, Y., Kinoshita, K., Akashi, T., Aoki, T. and Ayabe, S. (2002). „Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase”. Plant J. 31: 555—564. PMID 12207646.
- ^ Sawada, Y. & Ayabe, S. (2005). „Multiple mutagenesis of P450 isoflavonoid synthase reveals a key active-site residue”. Biochem. Biophys. Res. Commun. 330: 907—913. PMID 15809082.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- Isoflavonoid+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
