Lakto-N-biozidaza
| Lakto-N-biozidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.140 | ||||||||
| CAS broj | 146359-52-6 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Lakto-N-biozidaza (EC 3.2.1.140, Lacto-N-biosidase) je enzim sa sistematskim imenom oligosaharid lakto-N-biozilhidrolaza.[1][2] Ovaj enzim katalizuje sledeću hemijsku reakciju
- (1) beta-D-GaL-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-GaL-(1->4)-D-Glc + H2O beta-D-GaL-(1->3)-D-GlcNAc + beta-D-GaL-(1->4)-D-Glc
- (2) lakto-N-tetraoza + H2O lakto-N-bioza + laktoza
Enzim iz Streptomyces specifično hidrolizuje terminalne lakto-N-biozilne ostatake (beta-D-Gal-(1->3)-D-GlcNAc) iz neredukujućeg kraja oligosaharida sa strukturom beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).
Reference[уреди | уреди извор]
- ^ Sano, M., Hayakawa, K., Kato, I. (1992). „An enzyme releasing lacto-N-biose from oligosaccharides”. Proc. Natl. Acad. Sci. USA. 89: 8512—8516. PMID 1528855.
- ^ Sano, M., Hayakawa, K., Kato, I. (1993). „Purification and characterization of an enzyme releasing lacto-N-biose from oligosaccharides with type 1 chain”. J. Biol. Chem. 268: 18560—18566. PMID 7689556.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Lacto-N-biosidase на US National Library of Medicine Medical Subject Headings (MeSH)
