Ramnogalakturonanska hidrolaza
| Ramnogalakturonanska hidrolaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.2.1.171 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Ramnogalakturonanska hidrolaza (EC 3.2.1.171, ramnogalakturonaza A, RGaza A, RG-hidrolaza) je enzim sa sistematskim imenom ramnogalakturonan alfa-D-GalA-(1->2)-alfa-L-Rha hidrolaza.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Endohidroliza alfa-D-GalA-(1->2)-alfa-L-Rha glikozidne veze u ramnogalakturonan I osnovi sa inicijalnom inverzijom anomerne konfiguracije čime se odvajaju oligosaharidi sa beta-D-GalA ostatkom na redukujućem kraju
Ovaj enzim učestvuje u degradaciji ramnogalakturonana I kod Aspergillus aculeatus.
Reference[уреди | уреди извор]
- ^ Petersen, T.N., Kauppinen, S. and Larsen, S. (1997). „The crystal structure of rhamnogalacturonase A from Aspergillus aculeatus: a right-handed parallel β helix”. Structure. 5: 533—544. PMID 9115442.
- ^ Kofod, L.V., Kauppinen, S., Christgau, S., Andersen, L.N., Heldt-Hansen, H.P., Dorreich, K. and Dalboge, H. (1994). „Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus”. J. Biol. Chem. 269: 29182—29189. PMID 7961884.
- ^ Azadi, P., O'Neill, M.A., Bergmann, C., Darvill, A.G. and Albersheim, P. (1995). „The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase”. Glycobiology. 5: 783—789. PMID 8720076.
- ^ Petersen, T.N., Christgau, S., Kofod, L.V., Kauppinen, S., Johnson, A.H. and Larsen, S. (1997). „Crystallization and preliminary X-ray studies of rhamnogalacturonase A from Aspergillus aculeatus”. Acta Crystallogr. D Biol. Crystallogr. 53: 105—107. PMID 15299976.
- ^ Pitson, S.M., Mutter, M., van den Broek, L.A., Voragen, A.G. and Beldman, G. (1998). „Stereochemical course of hydrolysis catalysed by α-L-rhamnosyl and α-D-galacturonosyl hydrolases from Aspergillus aculeatus”. Biochem. Biophys. Res. Commun. 242: 552—559. PMID 9464254.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Rhamnogalacturonan+hydrolase на US National Library of Medicine Medical Subject Headings (MeSH)
