GNAQ

Guanin nukleotid-vezujući protein (G protein), q polipeptid
Guanin nukleotid-vezujući protein (G protein), q polipeptid
	PDB prikaz baziran na 2bcj.
Dostupne strukture
	2bcj
Identifikatori
Simboli	GNAQ; G-ALPHA-q; GAQ
Vanjski ID	OMIM: 600998 MGI: 95776 HomoloGene: 1566 GeneCards: GNAQ Gene
Ontologija gena
Molekularna funkcija	• vezivanje nukleotida; • aktivnost GTPaza; • aktivnost prenosnika signala; • GTP vezivanje;
Celularna komponenta	• citoplazma; • heterotrimerni G-proteinski kompleks; • membrana plazme;
Biološki proces	• skeletalni razvoj; • regulacija akcionog potencijala; • proteinska ADP-ribozilacija; • prenos signala; • signalni put G-protein spregnutog receptora; • G-protein signalizacija, aktivirajući put adenilat ciklaze; • aktivacija fosfolipaze C; • glutamatni signalni put; • razvoj srca; • koagulacija krvi; • neuronsko remodelovanje; • regulacija melanocitne diferencijacije; • pigmentacija tokom razvoja;
Pregled RNK izražavanja
	podaci
Ortolozi
Vrsta	Čovek
Entrez	2776
Ensembl	ENSG00000156052
UniProt	P50148
RefSeq (mRNA)	NM_002072
RefSeq (protein)	NP_002063
Lokacija (UCSC)	Chr 9:; 79.52 - 79.84 Mb
PubMed pretraga	[1]

GNAQ, Guanin nukleotid-vezujući protein G(q), podjedinica alfa, je protein koji je kod ljudi kodiran GNAQ genom.^[1]

Guanin nukleotid-vezujući proteini su familija heterotrimernih proteina koji se uparuju sa proteinima ćelijske površine, 7-TM domenskim receptorima, i posreduju intracelularnu signalizaciju. Aktivacija receptora katalizuje izmenu GDP za GTP. GDP je vezan za neaktivnu G protein alfa podedinicu. Vezivanje agonista dovodi do konformacione promene i disocijacije kompleksa. G protein alfa i beta-gama podjedinice imaju sposobnost regulacije niza celularnih efektora. Aktivacija se okončava GTPazom specifičnom za G-alfa podjedinicu. G-alfa-q podjedinica je jedan od heterotrimernih GTP-vezujućih proteina koji posreduje stimulaciju fosfolipaze C-beta (MIM 600230).^[2]

Interakcije[уреди | уреди извор]

Za GNAQ je bilo pokazano da interaguje sa natrijum-vodonik antiporterom 3, regulator 1,^[3] RIC8A,^[4]^[5] RGS16,^[6] beta adrenergički receptor kinazom,^[7] RGS4^[6]^[8] i Brutonovom tirozinskom kinazom.^[9]

Reference[уреди | уреди извор]

^ Dong Q, Shenker A, Way J, Haddad BR, Lin K, Hughes MR, McBride OW, Spiegel AM, Battey J (1997). „Molecular cloning of human G alpha q cDNA and chromosomal localization of the G alpha q gene (GNAQ) and a processed pseudogene”. Genomics. 30 (3): 470—75. PMID 8825633. doi:10.1006/geno.1995.1267.
^ „Entrez Gene: GNAQ guanine nucleotide binding protein (G protein), q polypeptide”.
^ Rochdi, Moulay Driss; Valérie, Watier; et al. (2002). „Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50)”. J. Biol. Chem. United States. 277 (43): 40751—9. ISSN 0021-9258. PMID 12193606. doi:10.1074/jbc.M207910200.
^ Klattenhoff, Carla; Martín, Montecino; et al. (2003). „Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol”. J. Cell. Physiol. United States. 195 (2): 151—7. ISSN 0021-9541. PMID 12652642. doi:10.1002/jcp.10300.
^ Tall, Gregory G.; Krumins Andrejs M; Gilman Alfred G (2003). „Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor”. J. Biol. Chem. United States. 278 (10): 8356—62. ISSN 0021-9258. PMID 12509430. doi:10.1074/jbc.M211862200.
^ ^а ^б
^ Day, Peter W.; Carman Christopher V; et al. (2003). „Differential interaction of GRK2 with members of the G alpha q family”. Biochemistry. United States. 42 (30): 9176—84. ISSN 0006-2960. PMID 12885252. doi:10.1021/bi034442.
^ Druey, K M.; Sullivan B M; et al. (1998). „Expression of GTPase-deficient Gialpha2 results in translocation of cytoplasmic RGS4 to the plasma membrane”. J. Biol. Chem. UNITED STATES. 273 (29): 18405—10. ISSN 0021-9258. PMID 9660808. doi:10.1074/jbc.273.29.18405.
^ Ma, Y C.; Huang X Y (1998). „Identification of the binding site for Gqalpha on its effector Bruton's tyrosine kinase”. Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 95 (21): 12197—201. ISSN 0027-8424. PMC 22808 . PMID 9770463. doi:10.1073/pnas.95.21.12197.

Literatura[уреди | уреди извор]

JR, Raymond; YV, Mukhin; A, Gelasco; et al. (2002). „Multiplicity of mechanisms of serotonin receptor signal transduction.”. Pharmacol. Ther. 92 (2-3): 179—212. PMID 11916537. doi:10.1016/S0163-7258(01)00169-3.
Van Oekelen D, Luyten WH, Leysen JE (2003). „5-HT2A and 5-HT2C receptors and their atypical regulation properties.”. Life Sci. 72 (22): 2429—49. PMID 12650852. doi:10.1016/S0024-3205(03)00141-3.
Lesch KP, Manji HK (1992). „Signal-transducing G proteins and antidepressant drugs: evidence for modulation of alpha subunit gene expression in rat brain.”. Biol. Psychiatry. 32 (7): 549—79. PMID 1333286. doi:10.1016/0006-3223(92)90070-G.
Thomas CP, Dunn MJ, Mattera R (1996). „Ca2+ signalling in K562 human erythroleukaemia cells: effect of dimethyl sulphoxide and role of G-proteins in thrombin- and thromboxane A2-activated pathways.”. Biochem. J. 312 ( Pt 1): 151—8. PMC 1136238 . PMID 7492305.
Blin N, Yun J, Wess J (1995). „Mapping of single amino acid residues required for selective activation of Gq/11 by the m3 muscarinic acetylcholine receptor.”. J. Biol. Chem. 270 (30): 17741—8. PMID 7629074. doi:10.1074/jbc.270.30.17741.
PS, Kabouridis; ST, Waters; S, Escobar; et al. (1995). „Expression of GTP-binding protein alpha subunits in human thymocytes.”. Mol. Cell. Biochem. 144 (1): 45—51. PMID 7791744. doi:10.1007/BF00926739.
A, Allgeier; S, Offermanns; Van Sande J; et al. (1994). „The human thyrotropin receptor activates G-proteins Gs and Gq/11.”. J. Biol. Chem. 269 (19): 13733—5. PMID 8188646.
PB, Wedegaertner; DH, Chu; PT, Wilson; et al. (1993). „Palmitoylation is required for signaling functions and membrane attachment of Gq alpha and Gs alpha.”. J. Biol. Chem. 268 (33): 25001—8. PMID 8227063.
Europe-Finner GN, Phaneuf S, Watson SP, López Bernal A (1993). „Identification and expression of G-proteins in human myometrium: up-regulation of G alpha s in pregnancy.”. Endocrinology. 132 (6): 2484—90. PMID 8504751. doi:10.1210/en.132.6.2484.
KL, Laugwitz; A, Allgeier; S, Offermanns; et al. (1996). „The human thyrotropin receptor: a heptahelical receptor capable of stimulating members of all four G protein families.”. Proc. Natl. Acad. Sci. U.S.A. 93 (1): 116—20. PMC 40189 . PMID 8552586. doi:10.1073/pnas.93.1.116.
SP, Denker; JM, McCaffery; GE, Palade; et al. (1996). „Differential distribution of alpha subunits and beta gamma subunits of heterotrimeric G proteins on Golgi membranes of the exocrine pancreas.”. J. Cell Biol. 133 (5): 1027—40. PMC 2120853 . PMID 8655576. doi:10.1083/jcb.133.5.1027.
Chen B, Leverette RD, Schwinn DA, Kwatra MM (1996). „Human G(alpha q): cDNA and tissue distribution.”. Biochim. Biophys. Acta. 1281 (2): 125—8. PMID 8664309. doi:10.1016/0005-2736(96)00039-9.
Johnson GJ, Leis LA, Dunlop PC (1996). „Specificity of G alpha q and G alpha 11 gene expression in platelets and erythrocytes. Expressions of cellular differentiation and species differences.”. Biochem. J. 318 ( Pt 3): 1023—31. PMC 1217719 . PMID 8836152.
J, Gomeza; S, Mary; I, Brabet; et al. (1996). „Coupling of metabotropic glutamate receptors 2 and 4 to G alpha 15, G alpha 16, and chimeric G alpha q/i proteins: characterization of new antagonists.”. Mol. Pharmacol. 50 (4): 923—30. PMID 8863838.
Petit A, Geoffroy P, Bélisle S (1997). „Expression of angiotensin II type-I receptor and phospholipase C-linked G alpha q/11 protein in the human placenta.”. J. Soc. Gynecol. Investig. 3 (6): 316—21. PMID 8923415. doi:10.1016/S1071-5576(96)00035-4.
Petit A, Geoffroy P, Bélisle S (1997). „Expression of G proteins in human placentas from pregnancies complicated by gestational hypertension.”. Life Sci. 60 (12): 953—60. PMID 9061052. doi:10.1016/S0024-3205(96)00654-6.
Kinsella BT, O'Mahony DJ, Fitzgerald GA (1997). „The human thromboxane A2 receptor alpha isoform (TP alpha) functionally couples to the G proteins Gq and G11 in vivo and is activated by the isoprostane 8-epi prostaglandin F2 alpha.”. J. Pharmacol. Exp. Ther. 281 (2): 957—64. PMID 9152406.
Wise A, Parenti M, Milligan G (1997). „Interaction of the G-protein G11alpha with receptors and phosphoinositidase C: the contribution of G-protein palmitoylation and membrane association.”. FEBS Lett. 407 (3): 257—60. PMID 9175863. doi:10.1016/S0014-5793(97)00300-1.
J, Gabbeta; X, Yang; MA, Kowalska; et al. (1997). „Platelet signal transduction defect with Galpha subunit dysfunction and diminished Galphaq in a patient with abnormal platelet responses.”. Proc. Natl. Acad. Sci. U.S.A. 94 (16): 8750—5. PMC 23110 . PMID 9238049. doi:10.1073/pnas.94.16.8750.

[pmid8825633-1] Dong Q, Shenker A, Way J, Haddad BR, Lin K, Hughes MR, McBride OW, Spiegel AM, Battey J (1997). „Molecular cloning of human G alpha q cDNA and chromosomal localization of the G alpha q gene (GNAQ) and a processed pseudogene”. Genomics. 30 (3): 470—75. PMID 8825633. doi:10.1006/geno.1995.1267.

[2] „Entrez Gene: GNAQ guanine nucleotide binding protein (G protein), q polypeptide”.

[pmid12193606-3] Rochdi, Moulay Driss; Valérie, Watier; et al. (2002). „Regulation of GTP-binding protein alpha q (Galpha q) signaling by the ezrin-radixin-moesin-binding phosphoprotein-50 (EBP50)”. J. Biol. Chem. United States. 277 (43): 40751—9. ISSN 0021-9258. PMID 12193606. doi:10.1074/jbc.M207910200.

[pmid12652642-4] Klattenhoff, Carla; Martín, Montecino; et al. (2003). „Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol”. J. Cell. Physiol. United States. 195 (2): 151—7. ISSN 0021-9541. PMID 12652642. doi:10.1002/jcp.10300.

[pmid12509430-5] Tall, Gregory G.; Krumins Andrejs M; Gilman Alfred G (2003). „Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor”. J. Biol. Chem. United States. 278 (10): 8356—62. ISSN 0021-9258. PMID 12509430. doi:10.1074/jbc.M211862200.

[pmid14634662-6] а ^б

[pmid12885252-7] Day, Peter W.; Carman Christopher V; et al. (2003). „Differential interaction of GRK2 with members of the G alpha q family”. Biochemistry. United States. 42 (30): 9176—84. ISSN 0006-2960. PMID 12885252. doi:10.1021/bi034442.

[pmid9660808-8] Druey, K M.; Sullivan B M; et al. (1998). „Expression of GTPase-deficient Gialpha2 results in translocation of cytoplasmic RGS4 to the plasma membrane”. J. Biol. Chem. UNITED STATES. 273 (29): 18405—10. ISSN 0021-9258. PMID 9660808. doi:10.1074/jbc.273.29.18405.

[pmid9770463-9] Ma, Y C.; Huang X Y (1998). „Identification of the binding site for Gqalpha on its effector Bruton's tyrosine kinase”. Proc. Natl. Acad. Sci. U.S.A. UNITED STATES. 95 (21): 12197—201. ISSN 0027-8424. PMC 22808 . PMID 9770463. doi:10.1073/pnas.95.21.12197.

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