Proteinska O-GlcNAkaza

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Proteinska O-GlcNAkaza
Identifikatori
EC broj 3.2.1.169
Baze podataka
IntEnz IntEnz pregled
BRENDA BRENDA pristup
ExPASy NiceZyme pregled
KEGG KEGG pristup
MetaCyc metabolički put
PRIAM profil
Strukture PBP RCSB PDB PDBe PDBsum

Proteinska O-GlcNAkaza (EC 3.2.1.169, OGA, glikozidna hidrolaza O-GlcNAkaza, O-GlcNAkaza, BtGH84, O-GlcNAc hidrolaza) je enzim sa sistematskim imenom (protein)-3-O-(N-acetil-D-glukozaminil)-L-serin/treonin N-acetilglukozaminil hidrolaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) [protein]-3-O-(N-acetil-beta-D-glukozaminil)-L-serin + H2O [protein]-L-serin + N-acetil-D-glukozamin
(2) [protein]-3-O-(N-acetil-beta-D-glukozaminil)-L-treonin + H2O [protein]-L-treonin + N-acetil-D-glukozamin

Kod viših eukariota posttranslaciona modifikacija proteinskih serina/treonina sa N-acetilglukozaminom (O-GlcNAc) je dinamička, induktivna i izobilna. Njome se regulišu mnogi ćelijski procesi putem ometanja proteinske fosforilacije.

Reference[уреди]

  1. Gao, Y., Wells, L., Comer, F.I., Parker, G.J. and Hart, G.W. (2001). „Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic β-N-acetylglucosaminidase from human brain”. J. Biol. Chem. 276: 9838—9845. PMID 11148210. 
  2. Wells, L., Gao, Y., Mahoney, J.A., Vosseller, K., Chen, C., Rosen, A. and Hart, G.W. (2002). „Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic β-N-acetylglucosaminidase, O-GlcNAcase”. J. Biol. Chem. 277: 1755—1761. PMID 11788610. 
  3. Cetinbas, N., Macauley, M.S., Stubbs, K.A., Drapala, R. and Vocadlo, D.J. (2006). „Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants”. Biochemistry. 45: 3835—3844. PMID 16533067. 
  4. Dennis, R.J., Taylor, E.J., Macauley, M.S., Stubbs, K.A., Turkenburg, J.P., Hart, S.J., Black, G.N., Vocadlo, D.J. and Davies, G.J. (2006). „Structure and mechanism of a bacterial β-glucosaminidase having O-GlcNAcase activity”. Nat. Struct. Mol. Biol. 13: 365—371. PMID 16565725. 
  5. Kim, E.J., Kang, D.O., Love, D.C. and Hanover, J.A. (2006). „Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate”. Carbohydr. Res. 341: 971—982. PMID 16584714. 
  6. Dong, D.L. & Hart, G.W. (1994). „Purification and characterization of an O-GlcNAc selective N-acetyl-β-D-glucosaminidase from rat spleen cytosol”. J. Biol. Chem. 269: 19321—19330. PMID 8034696. 

Literatura[уреди]

Spoljašnje veze[уреди]