Pseudomonalizin
| Pseudomonalizin | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.21.100 | ||||||||
| CAS broj | 848318-58-1 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Pseudomonalizin (EC 3.4.21.100, Pseudomonas sp. pepstatin-insenzitivna karboksilna proteinaza, pseudomonapepsin, sedolizin) je enzim.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Hidroliza B lanca insulina na -Glu13-Ala-, -Leu15-Tyr- i -Phe25-Tyr-, i angiotenzina I na -Tyr4-Ile-. Dobar sintetički supstrat je Lys-Pro-Ile-Glu-Phe-Phe(NO2)-Arg-Leu.
Ovaj enzim izlučuje Pseudomonas sp. No. 101. Optimalni pH je 4.
Reference[уреди | уреди извор]
- ^ Oda, K., Sugitani, M., Fukuhara, K. and Murao, S. (1987). „Purification and properties of a pepstatin-insensitive carboxyl proteinase from a Gram-negative bacterium”. Biochim. Biophys. Acta. 923: 463—469. PMID 3548827.
- ^ Oda, K., Nakatani, H. and Dunn, B.M. (1992). „Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101”. Biochim. Biophys. Acta. 1120: 208—214. PMID 1562589.
- ^ Wlodawer, A., Li, M., Dauter, Z., Gustchina, A., Uchida, K., Oyama, H., Dunn, B.M. and Oda, K. (2001). „Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes”. Nat. Struct. Biol. 8: 442—446. PMID 11323721.
- ^ Wlodawer, A., Li, M., Gustchina, A., Oyama, H., Dunn, B.M. and Oda, K. (2003). „Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases”. Acta Biochim. Pol. 50: 81—102. PMID 12673349.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
