23S rRNK (uracil1939-C5)-metiltransferaza
(преусмерено са RumA)
| 23S rRNK (uracil1939-C5)-metiltransferaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 2.1.1.190 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
23S rRNK (uracil1939-C5)-metiltransferaza (EC 2.1.1.190, RumA, RNK uridinska metiltransferaza A, YgcA) je enzim sa sistematskim imenom S-adenozil-L-metionin:23S rRNK (uracil1939-C5)-metiltransferaza.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
- S-adenozil-L-metionin + uracil1939 u 23S rRNK S-adenozil-L-homocistein + 5-metiluracil1939 u 23S rRNK
Ovaj enzim specifično metiliše uracil1939 u C5 poziciji 23S rRNK. Enzim sadrži [4Fe-4S] klaster koji je koordinisan sa četiri konzervisana cisteinska ostataka.
Reference[уреди | уреди извор]
- ^ Agarwalla, S., Kealey, J.T., Santi, D.V. and Stroud, R.M. (2002). „Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli”. J. Biol. Chem. 277: 8835—8840. PMID 11779873.
- ^ Lee, T.T., Agarwalla, S. and Stroud, R.M. (2004). „Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase”. Structure. 12: 397—407. PMID 15016356.
- ^ Madsen, C.T., Mengel-Jorgensen, J., Kirpekar, F. and Douthwaite, S. (2003). „Identifying the methyltransferases for m5U747 and m5U1939 in 23S rRNA using MALDI mass spectrometry”. Nucleic Acids Res. 31: 4738—4746. PMID 12907714.
- ^ Persaud, C., Lu, Y., Vila-Sanjurjo, A., Campbell, J.L., Finley, J. and O'Connor, M. (2010). „Mutagenesis of the modified bases, m5U1939 and Ψ2504, in Escherichia coli 23S rRNA”. Biochem. Biophys. Res. Commun. 392: 223—227. PMID 20067766.
- ^ Agarwalla, S., Stroud, R.M. and Gaffney, B.J. (2004). „Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies”. J. Biol. Chem. 279: 34123—34129. PMID 15181002.
- ^ Lee, T.T., Agarwalla, S. and Stroud, R.M. (2005). „A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function”. Cell. 120: 599—611. PMID 15766524.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- 23S+rRNA+(uracil1939-C5)-methyltransferase на US National Library of Medicine Medical Subject Headings (MeSH)
