Skvalen sintaza
| Skvalen sintaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
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| Identifikatori | |||||||||
| EC broj | 2.5.1.21 | ||||||||
| CAS broj | 9077-14-9 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
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Skvalen sintaza (EC 2.5.1.21, farneziltransferaza, preskvalen-difosfatna sintaza, preskvalenska sintaza, skvalenska sintetaza, farnezil-difosfatna farneziltransferaza, SQS) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat:(2E,6E)-farnezil-difosfat farneziltransferaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju
- 2 (2E,6E)-farnezil difosfat + NAD(P)H + H+ skvalen + 2 difosfat + NAD(P)+ (sveukupna reakcija)
- (1a) 2 (2E,6E)-farnezil difosfat difosfat + preskvalen difosfat
- (1b) preskvalen difosfat + NAD(P)H + H+ skvalen + difosfat + NAD(P)+
Ovaj mikrozomalni enzim katalizuje privi korak biosinteze sterola.
Reference[уреди | уреди извор]
- ^ Kuswick-Rabiega, G. & Rilling, H.C. (1987). „Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities”. J. Biol. Chem. 262: 1505—1509. PMID 3805037.
- ^ Ericsson, J., Appelkvist, E.L., Thelin, A., Chojnacki, T. and Dallner, G. (1992). „Isoprenoid biosynthesis in rat liver peroxisomes. Characterization of cis-prenyltransferase and squalene synthetase”. J. Biol. Chem. 267: 18708—18714. PMID 1527001.
- ^ Tansey, T.R. & Shechter, I. (2000). „Structure and regulation of mammalian squalene synthase”. Biochim. Biophys. Acta. 1529: 49—62. PMID 11111077.
- ^ LoGrasso, P.V., Soltis, D.A. and Boettcher, B.R. (1993). „Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase”. Arch. Biochem. Biophys. 307: 193—199. PMID 8239656.
- ^ Shechter, I., Klinger, E., Rucker, M.L., Engstrom, R.G., Spirito, J.A., Islam, M.A., Boettcher, B.R. and Weinstein, D.B. (1992). „Solubilization, purification, and characterization of a truncated form of rat hepatic squalene synthetase”. J. Biol. Chem. 267: 8628—8635. PMID 1569107.
- ^ Agnew, W.S. and Popják, G. (1978). „Squalene synthetase. Stoichiometry and kinetics of presqualene pyrophosphate and squalene synthesis by yeast microsomes”. J. Biol. Chem. 253: 4566—4573. PMID 26684.
- ^ Pandit, J., Danley, D.E., Schulte, G.K., Mazzalupo, S., Pauly, T.A., Hayward, C.M., Hamanaka, E.S., Thompson, J.F. and Harwood, H.J., Jr. (2000). „Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis”. J. Biol. Chem. 275: 30610—30617. PMID 10896663.
- ^ Radisky, E.S. & Poulter, C.D. (2000). „Squalene synthase: steady-state, pre-steady-state, and isotope-trapping studies”. Biochemistry. 39: 1748—1760. PMID 10677224.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze[уреди | уреди извор]
- Squalene+synthase на US National Library of Medicine Medical Subject Headings (MeSH)
