Skvalen sintaza

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Skvalen sintaza
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Identifikatori
EC broj 2.5.1.21
CAS broj 9077-14-9
Baze podataka
IntEnz IntEnz pregled
BRENDA BRENDA pristup
ExPASy NiceZyme pregled
KEGG KEGG pristup
MetaCyc metabolički put
PRIAM profil
Strukture PBP RCSB PDB PDBe PDBsum

Skvalen sintaza (EC 2.5.1.21, farneziltransferaza, preskvalen-difosfatna sintaza, preskvalenska sintaza, skvalenska sintetaza, farnezil-difosfatna farneziltransferaza, SQS) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat:(2E,6E)-farnezil-difosfat farneziltransferaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

2 (2E,6E)-farnezil difosfat + NAD(P)H + H+ skvalen + 2 difosfat + NAD(P)+ (sveukupna reakcija)
(1a) 2 (2E,6E)-farnezil difosfat difosfat + preskvalen difosfat
(1b) preskvalen difosfat + NAD(P)H + H+ skvalen + difosfat + NAD(P)+

Ovaj mikrozomalni enzim katalizuje privi korak biosinteze sterola.

Reference[уреди]

  1. Kuswick-Rabiega, G. & Rilling, H.C. (1987). „Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities”. J. Biol. Chem. 262: 1505—1509. PMID 3805037. 
  2. Ericsson, J., Appelkvist, E.L., Thelin, A., Chojnacki, T. and Dallner, G. (1992). „Isoprenoid biosynthesis in rat liver peroxisomes. Characterization of cis-prenyltransferase and squalene synthetase”. J. Biol. Chem. 267: 18708—18714. PMID 1527001. 
  3. Tansey, T.R. & Shechter, I. (2000). „Structure and regulation of mammalian squalene synthase”. Biochim. Biophys. Acta. 1529: 49—62. PMID 11111077. 
  4. LoGrasso, P.V., Soltis, D.A. and Boettcher, B.R. (1993). „Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase”. Arch. Biochem. Biophys. 307: 193—199. PMID 8239656. 
  5. Shechter, I., Klinger, E., Rucker, M.L., Engstrom, R.G., Spirito, J.A., Islam, M.A., Boettcher, B.R. and Weinstein, D.B. (1992). „Solubilization, purification, and characterization of a truncated form of rat hepatic squalene synthetase”. J. Biol. Chem. 267: 8628—8635. PMID 1569107. 
  6. Agnew, W.S. and Popják, G. (1978). „Squalene synthetase. Stoichiometry and kinetics of presqualene pyrophosphate and squalene synthesis by yeast microsomes”. J. Biol. Chem. 253: 4566—4573. PMID 26684. 
  7. Pandit, J., Danley, D.E., Schulte, G.K., Mazzalupo, S., Pauly, T.A., Hayward, C.M., Hamanaka, E.S., Thompson, J.F. and Harwood, H.J., Jr. (2000). „Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis”. J. Biol. Chem. 275: 30610—30617. PMID 10896663. 
  8. Radisky, E.S. & Poulter, C.D. (2000). „Squalene synthase: steady-state, pre-steady-state, and isotope-trapping studies”. Biochemistry. 39: 1748—1760. PMID 10677224. 

Literatura[уреди]

Spoljašnje veze[уреди]