Skvalen sintaza

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Skvalen sintaza
3q30.png
Identifikatori
EC broj 2.5.1.21
CAS broj 9077-14-9
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Skvalen sintaza (EC 2.5.1.21, farneziltransferaza, preskvalen-difosfatna sintaza, preskvalenska sintaza, skvalenska sintetaza, farnezil-difosfatna farneziltransferaza, SQS) je enzim sa sistematskim imenom (2E,6E)-farnezil-difosfat:(2E,6E)-farnezil-difosfat farneziltransferaza.[1][2][3][4][5][6][7][8] Ovaj enzim katalizuje sledeću hemijsku reakciju

2 (2E,6E)-farnezil difosfat + NAD(P)H + H+ \rightleftharpoons skvalen + 2 difosfat + NAD(P)+ (sveukupna reakcija)
(1a) 2 (2E,6E)-farnezil difosfat \rightleftharpoons difosfat + preskvalen difosfat
(1b) preskvalen difosfat + NAD(P)H + H+ \rightleftharpoons skvalen + difosfat + NAD(P)+

Ovaj mikrozomalni enzim katalizuje privi korak biosinteze sterola.

Reference[уреди]

  1. Kuswick-Rabiega, G. and Rilling, H.C. (1987). „Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities”. J. Biol. Chem. 262: 1505-1509. PMID 3805037. 
  2. Ericsson, J., Appelkvist, E.L., Thelin, A., Chojnacki, T. and Dallner, G. (1992). „Isoprenoid biosynthesis in rat liver peroxisomes. Characterization of cis-prenyltransferase and squalene synthetase”. J. Biol. Chem. 267: 18708-18714. PMID 1527001. 
  3. Tansey, T.R. and Shechter, I. (2000). „Structure and regulation of mammalian squalene synthase”. Biochim. Biophys. Acta 1529: 49-62. PMID 11111077. 
  4. LoGrasso, P.V., Soltis, D.A. and Boettcher, B.R. (1993). „Overexpression, purification, and kinetic characterization of a carboxyl-terminal-truncated yeast squalene synthetase”. Arch. Biochem. Biophys. 307: 193-199. PMID 8239656. 
  5. Shechter, I., Klinger, E., Rucker, M.L., Engstrom, R.G., Spirito, J.A., Islam, M.A., Boettcher, B.R. and Weinstein, D.B. (1992). „Solubilization, purification, and characterization of a truncated form of rat hepatic squalene synthetase”. J. Biol. Chem. 267: 8628-8635. PMID 1569107. 
  6. Agnew, W.S. and Popják, G. (1978). „Squalene synthetase. Stoichiometry and kinetics of presqualene pyrophosphate and squalene synthesis by yeast microsomes”. J. Biol. Chem. 253: 4566-4573. PMID 26684. 
  7. Pandit, J., Danley, D.E., Schulte, G.K., Mazzalupo, S., Pauly, T.A., Hayward, C.M., Hamanaka, E.S., Thompson, J.F. and Harwood, H.J., Jr. (2000). „Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis”. J. Biol. Chem. 275: 30610-30617. PMID 10896663. 
  8. Radisky, E.S. and Poulter, C.D. (2000). „Squalene synthase: steady-state, pre-steady-state, and isotope-trapping studies”. Biochemistry 39: 1748-1760. PMID 10677224. 

Literatura[уреди]

Spoljašnje veze[уреди]