(S)-hidroksinitrilna lijaza

Из Википедије, слободне енциклопедије
(S)-hidroksinitrilna lijaza
EC broj
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures

(S)-hidroksinitrilna lijaza (EC, (S)-cijanohidrin produkujuća hidroksinitrilna lijaza, (S)-oksinitrilaza, (S)-HbHNL, (S)-MeHNL, hidroksinitrilna lijaza, oksinitrilaza, HbHNL, MeHNL, (S)-selektivna hidroksinitrilna lijaza, (S)-cijanohidrin karbonil-lijaza (formira cijanid)) je enzim sa sistematskim imenom (S)-cijanohidrin lijaza (formira cijanid).[1][2][3][4][5][6][7][8][9][10] Ovaj enzim katalizuje sledeću hemijsku reakciju

(1) alifatični (S)-hidroksinitril \rightleftharpoons cijanid + alifatični aldehid ili keton
(2) aromatična (S)-hidroksinitril \rightleftharpoons cijanid + aromatični aldehid

Ovaj enzim katalizuje razlaganje hidroksinitrila u cijanid i korespondirajući aldehid ili keton.


  1. Förster, S., Roos, J., Effenberger, F., Wajant, H. and Sprauer, A. (1996). „The first recombinant hydroxynitrile lyase and its application in the synthesis of (S)-cyanohydrins”. Angew. Chem. Int. Ed. 35: 437-439. 
  2. Bühler, H., Effenberger, F., Förster, S., Roos, J. and Wajant, H. (2003). „Substrate specificity of mutants of the hydroxynitrile lyase from Manihot esculenta”. Chembiochem. 4: 211-216. PMID 12616635. 
  3. Semba, H., Dobashi, Y. and Matsui, T. (2008). „Expression of hydroxynitrile lyase from Manihot esculenta in yeast and its application in (S)-mandelonitrile production using an immobilized enzyme reactor”. Biosci. Biotechnol. Biochem. 72: 1457-1463. PMID 18540112. 
  4. Avi, M., Wiedner, R.M., Griengl, H. and Schwab, H. (2008). „Improvement of a stereoselective biocatalytic synthesis by substrate and enzyme engineering: 2-hydroxy-(4′-oxocyclohexyl)acetonitrile as the model”. Chemistry 14: 11415-11422. PMID 19006143. 
  5. von Langermann, J., Guterl, J.K., Pohl, M., Wajant, H. and Kragl, U. (2008). „Hydroxynitrile lyase catalyzed cyanohydrin synthesis at high pH-values”. Bioprocess Biosyst. Eng. 31: 155-161. PMID 18204865. 
  6. Schmidt, A., Gruber, K., Kratky, C. and Lamzin, V.S. (2008). „Atomic resolution crystal structures and quantum chemistry meet to reveal subtleties of hydroxynitrile lyase catalysis”. J. Biol. Chem. 283: 21827-21836. PMID 18524775. 
  7. Gartler, G., Kratky, C. and Gruber, K. (2007). „Structural determinants of the enantioselectivity of the hydroxynitrile lyase from Hevea brasiliensis”. J. Biotechnol. 129: 87-97. PMID 17250917. 
  8. Wagner, U.G., Schall, M., Hasslacher, M., Hayn, M., Griengl, H., Schwab, H. and Kratky, C. (1996). „Crystallization and preliminary X-ray diffraction studies of a hydroxynitrile lyase from Hevea brasiliensis”. Acta Crystallogr. D Biol. Crystallogr. 52: 591-593. PMID 15299689. 
  9. Schmidt, M., Herve, S., Klempier, N. and Griengl, H. (1996). „Preparation of optically active cyanohydrins using the (S)-hydroxynitrile lyase from Hevea brasiliensis”. Tetrahedron 52: 7833-7840. 
  10. Klempier, N. and Griengl, H. (1993). „Aliphatic (S)-cyanohydrins by enzyme catalyzed synthesis”. Tetrahedron Lett. 34: 4769-4772. 


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