Pektinska lijaza
| Pektinska lijaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 4.2.2.10 | ||||||||
| CAS broj | 9033-35-6 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
Pektinska lijaza (EC 4.2.2.10, pectin trans-eliminaza, endo-pektin lijaza, polimetilgalacturonska transeliminaza, pectin metiltranseliminaza, pectolijaza, PL, PNL, PMGL) je enzim sa sistematskim imenom (1->4)-6-O-metil-alfa-D-galakturonan lijaza.[1][2][3][4] Ovaj enzim katalizuje sledeću hemijsku reakciju
- Eliminativno razlaganje (1->4)-alfa-D-galakturonan metil estra čime se formiraju oligosaharidi sa 4-dezoksi-6-O-metil-alfa-D-galakt-4-enuronozil grupama na njihovim neredukujućim krajevima
Ovaj enzim je reaktivniji na pektinu u odnosu na pectat.
Reference[уреди | уреди извор]
- ^ Albersheim, P., Neukom, H. and Deuel, H. (1960). „Über die Bildung von ungesättigten Abbauprodukten durch ein pekinabbauendes Enzym”. Helv. Chim. Acta. 43: 1422—1426.
- ^ Mayans, O., Scott, M., Connerton, I., Gravesen, T., Benen, J., Visser, J., Pickersgill, R. and Jenkins, J. (1997). „Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases”. Structure. 5: 677—689. PMID 9195887.
- ^ Kester, H.C.M & Visser, J. (1994). „Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger”. FEMS Microbiol. Lett. 120: 63—68.
- ^ Mutenda, K.E., Körner, R., Christensen, T.M.I.E., Mikkelsen, J. and Roepstorff, P. (2002). „Application of mass spectrometry to determine the activity and specificity of pectin lyase A”. Carbohydr. Res. 337: 1213—1223. PMID 12110197.
Literatura[уреди | уреди извор]
- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze[уреди | уреди извор]
- Pectin+lyase на US National Library of Medicine Medical Subject Headings (MeSH)
