Peptid-metionin (R)-S-oksid reduktaza

Из Википедије, слободне енциклопедије
Peptid-metionin (R)-S-oksid reduktaza
EC broj
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures

Peptid-metionin (R)-S-oksid reduktaza (EC, MsrB, metionin sulfoksidna reduktaza (nespecifična), pMSR, metionin S-oksidna reduktaza (nespecifična), selenoprotein R, metionin S-oksidna reduktaza (oksiduje R-formu), metionin sulfoksid reduktaza B, SelR, SelX, PilB, pRMsr) je enzim sa sistematskim imenom peptid-metionin:tioredoksin-disulfid S-oksidoreduktaza (formira metionin (R)-S-oksid).[1][2][3][4][5][6][7][8][9][10] Ovaj enzim katalizuje sledeću hemijsku reakciju

peptid-L-metionin + tioredoksin disulfid +H2O peptid-L-metionin (R)-S-oksid + tioredoksin

Ova reakcija teče u reverznom smeru. Enzim manifestuje visoku specifičnost za redukciju R-forme L-metionin S-oksida. On brže deluje na L-metionin S-oksid nego na D-metionin S-oksid. Aktivnost je veća na ostataku peptida nego na slobodnoj aminokiselini.


  1. Moskovitz, J., Singh, V.K., Requena, J., Wilkinson, B.J., Jayaswal, R.K. and Stadtman, E.R. (2002). „Purification and characterization of methionine sulfoxide reductases from mouse and Staphylococcus aureus and their substrate stereospecificity”. Biochem. Biophys. Res. Commun. 290: 62-65. PMID 11779133 
  2. Taylor, A.B., Benglis, D.M., Jr., Dhandayuthapani, S. and Hart, P.J. (2003). „Structure of Mycobacterium tuberculosis methionine sulfoxide reductase A in complex with protein-bound methionine”. J. Bacteriol. 185: 4119-4126. PMID 12837786 
  3. Singh, V.K. and Moskovitz, J. (2003). „Multiple methionine sulfoxide reductase genes in Staphylococcus aureus: expression of activity and roles in tolerance of oxidative stress”. Microbiology 149: 2739-2747. PMID 14523107 
  4. Boschi-Muller, S., Olry, A., Antoine, M. and Branlant, G. (2005). „The enzymology and biochemistry of methionine sulfoxide reductases”. Biochim. Biophys. Acta 1703: 231-238. PMID 15680231 
  5. Ezraty, B., Aussel, L. and Barras, F. (2005). „Methionine sulfoxide reductases in prokaryotes”. Biochim. Biophys. Acta 1703: 221-229. PMID 15680230 
  6. Weissbach, H., Resnick, L. and Brot, N. (2005). „Methionine sulfoxide reductases: history and cellular role in protecting against oxidative damage”. Biochim. Biophys. Acta 1703: 203-212. PMID 15680228 
  7. Kauffmann, B., Aubry, A. and Favier, F. (2005). „The three-dimensional structures of peptide methionine sulfoxide reductases: current knowledge and open questions”. Biochim. Biophys. Acta 1703: 249-260. PMID 15680233 
  8. Vougier, S., Mary, J. and Friguet, B. (2003). „Subcellular localization of methionine sulphoxide reductase A (MsrA): evidence for mitochondrial and cytosolic isoforms in rat liver cells”. Biochem. J. 373: 531-537. PMID 12693988 
  9. Olry, A., Boschi-Muller, S., Marraud, M., Sanglier-Cianferani, S., Van Dorsselear, A. and Branlant, G. (2002). „Characterization of the methionine sulfoxide reductase activities of PILB, a probable virulence factor from Neisseria meningitidis”. J. Biol. Chem. 277: 12016-12022. PMID 11812798 
  10. Sagher, D., Brunell, D., Hejtmancik, J.F., Kantorow, M., Brot, N. and Weissbach, H. (2006). „Thionein can serve as a reducing agent for the methionine sulfoxide reductases”. Proc. Natl. Acad. Sci. USA 103: 8656-8661. PMID 16735467 


Spoljašnje veze[уреди]