Хидролиза

Генерички механизам за хидролошку реакцију

Хидролиза је хемијска реакција која се заснива на распаду молекула хемијских једињења на два мања фрагмента под утицајем контакта са водом или воденом паром. The term is used broadly for substitution, elimination, and solvation reactions in which water is the nucleophile. У посебном случају хидролизе соли, то је реакција јона са водом (углавном се ствара кисела или базна средина). Хидролиза је посебна грана солволизе.

Biological hydrolysis is the cleavage of biomolecules where a water molecule is consumed to effect the separation of a larger molecule into component parts. When a carbohydrate is broken into its component sugar molecules by hydrolysis (e.g., sucrose being broken down into glucose and fructose), this is recognized as saccharification.

Hydrolysis reactions can be the reverse of a condensation reaction in which two molecules join into a larger one and eject a water molecule. Thus hydrolysis adds water to break down, whereas condensation builds up by removing water.

Типови

Usually hydrolysis is a chemical process in which a molecule of water is added to a substance. Sometimes this addition causes both substance and water molecule to split into two parts. In such reactions, one fragment of the target molecule (or parent molecule) gains a hydrogen ion. It breaks a chemical bond in the compound.

Salts

A common kind of hydrolysis occurs when a salt of a weak acid or weak base (or both) is dissolved in water. Water spontaneously ionizes into hydroxide anions and hydronium cations. The salt also dissociates into its constituent anions and cations. For example, sodium acetate dissociates in water into sodium and acetate ions. Sodium ions react very little with the hydroxide ions whereas the acetate ions combine with hydronium ions to produce acetic acid. In this case the net result is a relative excess of hydroxide ions, yielding a basic solution.

Strong acids also undergo hydrolysis. For example, dissolving sulfuric acid (H₂SO₄) in water is accompanied by hydrolysis to give hydronium and bisulfate, the sulfuric acid's conjugate base. For a more technical discussion of what occurs during such a hydrolysis, see Brønsted–Lowry acid–base theory.

Esters and amides

Acid–base-catalysed hydrolyses are very common; one example is the hydrolysis of amides or esters. Their hydrolysis occurs when the nucleophile (a nucleus-seeking agent, e.g., water or hydroxyl ion) attacks the carbon of the carbonyl group of the ester or amide. In an aqueous base, hydroxyl ions are better nucleophiles than polar molecules such as water. In acids, the carbonyl group becomes protonated, and this leads to a much easier nucleophilic attack. The products for both hydrolyses are compounds with carboxylic acid groups.

Perhaps the oldest commercially practiced example of ester hydrolysis is saponification (formation of soap). It is the hydrolysis of a triglyceride (fat) with an aqueous base such as sodium hydroxide (NaOH). During the process, glycerol is formed, and the fatty acids react with the base, converting them to salts. These salts are called soaps, commonly used in households.

In addition, in living systems, most biochemical reactions (including ATP hydrolysis) take place during the catalysis of enzymes. The catalytic action of enzymes allows the hydrolysis of proteins, fats, oils, and carbohydrates. As an example, one may consider proteases (enzymes that aid digestion by causing hydrolysis of peptide bonds in proteins). They catalyze the hydrolysis of interior peptide bonds in peptide chains, as opposed to exopeptidases (another class of enzymes, that catalyze the hydrolysis of terminal peptide bonds, liberating one free amino acid at a time).

However, proteases do not catalyze the hydrolysis of all kinds of proteins. Their action is stereo-selective: Only proteins with a certain tertiary structure are targeted as some kind of orienting force is needed to place the amide group in the proper position for catalysis. The necessary contacts between an enzyme and its substrates (proteins) are created because the enzyme folds in such a way as to form a crevice into which the substrate fits; the crevice also contains the catalytic groups. Therefore, proteins that do not fit into the crevice will not undergo hydrolysis. This specificity preserves the integrity of other proteins such as hormones, and therefore the biological system continues to function normally.

Mechanism for acid-catalyzed hydrolysis of an amide.

Upon hydrolysis, an amide converts into a carboxylic acid and an amine or ammonia (which in the presence of acid are immediately converted to ammonium salts). One of the two oxygen groups on the carboxylic acid are derived from a water molecule and the amine (or ammonia) gains the hydrogen ion. The hydrolysis of peptides gives amino acids.

Many polyamide polymers such as nylon 6,6 hydrolyze in the presence of strong acids. The process leads to depolymerization. For this reason nylon products fail by fracturing when exposed to small amounts of acidic water. Polyesters are also susceptible to similar polymer degradation reactions. The problem is known as environmental stress cracking.

ATP

Hydrolysis is related to energy metabolism and storage. All living cells require a continual supply of energy for two main purposes: the biosynthesis of micro and macromolecules, and the active transport of ions and molecules across cell membranes. The energy derived from the oxidation of nutrients is not used directly but, by means of a complex and long sequence of reactions, it is channeled into a special energy-storage molecule, adenosine triphosphate (ATP). The ATP molecule contains pyrophosphate linkages (bonds formed when two phosphate units are combined) that release energy when needed. ATP can undergo hydrolysis in two ways: Firstly, the removal of terminal phosphate to form adenosine diphosphate (ADP) and inorganic phosphate, with the reaction:

ATP + H
2O → ADP + P_i

Secondly, the removal of a terminal diphosphate to yield adenosine monophosphate (AMP) and pyrophosphate. The latter usually undergoes further cleavage into its two constituent phosphates. This results in biosynthesis reactions, which usually occur in chains, that can be driven in the direction of synthesis when the phosphate bonds have undergone hydrolysis.

Полисахариди

Сахароза. The glycoside bond is represented by the central oxygen atom, which holds the two monosaccharide units together.

Monosaccharides can be linked together by glycosidic bonds, which can be cleaved by hydrolysis. Two, three, several or many monosaccharides thus linked form disaccharides, trisaccharides, oligosaccharides, or polysaccharides, respectively. Enzymes that hydrolyze glycosidic bonds are called "glycoside hydrolases" or "glycosidases".

The best-known disaccharide is sucrose (table sugar). Hydrolysis of sucrose yields glucose and fructose. Invertase is a sucrase used industrially for the hydrolysis of sucrose to so-called invert sugar. Lactase is essential for digestive hydrolysis of lactose in milk; many adult humans do not produce lactase and cannot digest the lactose in milk.

The hydrolysis of polysaccharides to soluble sugars can be recognized as saccharification. Malt made from barley is used as a source of β-amylase to break down starch into the disaccharide maltose, which can be used by yeast to produce beer. Other amylase enzymes may convert starch to glucose or to oligosaccharides. Cellulose is first hydrolyzed to cellobiose by cellulase and then cellobiose is further hydrolyzed to glucose by beta-glucosidase. Ruminants such as cows are able to hydrolyze cellulose into cellobiose and then glucose because of symbiotic bacteria that produce cellulases.

Metal aqua ions

Главни чланак: Metal ions in aqueous solution

Metal ions are Lewis acids, and in aqueous solution they form metal aquo complexes of the general formula M(H₂O)_n^m+. The aqua ions undergo hydrolysis, to a greater or lesser extent. The first hydrolysis step is given generically as

M(H₂O)_n^m+ + H₂O ⇌ M(H₂O)_n−1(OH)^(m−1)+ + H₃O⁺

Thus the aqua cations behave as acids in terms of Brønsted–Lowry acid–base theory. This effect is easily explained by considering the inductive effect of the positively charged metal ion, which weakens the O-H bond of an attached water molecule, making the liberation of a proton relatively easy.

The dissociation constant, pK_a, for this reaction is more or less linearly related to the charge-to-size ratio of the metal ion. Ions with low charges, such as Na⁺ are very weak acids with almost imperceptible hydrolysis. Large divalent ions such as Ca²⁺, Zn²⁺, Sn²⁺ and Pb²⁺ have a pK_a of 6 or more and would not normally be classed as acids, but small divalent ions such as Be²⁺ undergo extensive hydrolysis. Trivalent ions like Al³⁺ and Fe³⁺ are weak acids whose pK_a is comparable to that of acetic acid. Solutions of salts such as BeCl₂ or Al(NO₃)₃ in water are noticeably acidic; the hydrolysis can be suppressed by adding an acid such as nitric acid, making the solution more acidic.

Hydrolysis may proceed beyond the first step, often with the formation of polynuclear species via the process of olation. Some "exotic" species such as Sn₃(OH)₄²⁺ are well characterized. Hydrolysis tends to proceed as pH rises leading, in many cases, to the precipitation of a hydroxide such as Al(OH)₃ or AlO(OH). These substances, major constituents of bauxite, are known as laterites and are formed by leaching from rocks of most of the ions other than aluminium and iron and subsequent hydrolysis of the remaining aluminium and iron.

Реакције хидролизе

Обично се реакција хидролизе одвија по општем шаблону:

A-B + H₂O → A-H + B-OH

мада постоји могућност и за сложеније механизме те реакције, нпр.

A-B + 2 H₂O → A-OH + B-OH + H₂

које се одигравају у гасовитој фази при високим температурама, или у условима електролизе

Хидролиза је супротан процес од хидролитичке кондензације - тј. реакције спајања два или више молекула са издвајањем молекула воде.

Многе реакције хидролизе су повратне, при чему смер реакције зависи од њених услова:

Нпр. хидролиза естара:

RCOOR' + H₂O → RCOOH + R'OH

захтева додавање одређене количине воде у реакциони систем; уколико нема довољно воде настаје реакција кондензације тек настале киселине (RCOOH) и алкохола (OH), која је у ствари иста као и претходна само супротног смера:

RCOOH + R'OH → RCOOR' + H₂O

Реакција хидролизе није исто што и Електролитичка дисоцијација.

Дисоцијација се заснива на распаду молекула под дејством растварача (растварач може бити и вода) али без грађења ковалентних веза са њим, док се хидратација заснива на стварању комплексних једињења са водом везаних водоничном везом.

Види још

• Натријум-стеарат

Литература

• Jia, Chengguo; Kitamura, Tsugio; Fujiwara, Yuzo (2001). „Catalytic Functionalization of Arenes and Alkanes via C−H Bond Activation”. Accounts of Chemical Research. 34 (8): 633—639. PMID 11513570. doi:10.1021/ar000209h. 
• Kolb, Hartmuth C.; Vannieuwenhze, Michael S.; Sharpless, K. Barry (1994). „Catalytic Asymmetric Dihydroxylation”. Chemical Reviews. 94 (8): 2483—2547. doi:10.1021/cr00032a009. 
• Huang, X.; Groves, J. T. (2017). „Beyond ferryl-mediated hydroxylation: 40 years of the rebound mechanism and C–H activation”. JBIC Journal of Biological Inorganic Chemistry. 22 (2–3): 185—207. PMC 5350257 . PMID 27909920. doi:10.1007/s00775-016-1414-3. 
• Zurlo, Giada; Guo, Jianping; Takada, Mamoru; Wei, Wenyi; Zhang, Qing (децембар 2016). „New Insights into Protein Hydroxylation and Its Important Role in Human Diseases”. Biochimica et Biophysica Acta (BBA) - Reviews on Cancer. 1866 (2): 208—220. ISSN 0006-3002. PMC 5138100 . PMID 27663420. doi:10.1016/j.bbcan.2016.09.004. CS1 одржавање: Формат датума (веза)
• T. Shantha Raju (2019), „Hydroxylation of Proteins”, Co- and Post-Translational Modifications of Therapeutic Antibodies and Proteins (на језику: енглески), John Wiley & Sons, стр. 119—131, ISBN 978-1-119-05335-4, doi:10.1002/9781119053354.ch10 
• Holmgren, Steven K; Bretscher, Lynn E; Taylor, Kimberly M; Raines, Ronald T (1999). „A hyperstable collagen mimic”. Chemistry & Biology. 6 (2): 63—70. PMID 10021421. doi:10.1016/S1074-5521(99)80003-9 . 
• Hausinger RP (2004). „Fe(II)/α-ketoglutarate-dependent hydroxylases and related enzymes”. Crit. Rev. Biochem. Mol. Biol. 39 (1): 21—68. PMID 15121720. S2CID 85784668. doi:10.1080/10409230490440541. 
• Voet, Donald; Voet, Judith G.; Pratt, Charlotte W. (2016). Principles of Biochemistry. Wiley. стр. 143. ISBN 978-1-119-45166-2.